Adachi Y, Takano E, Murachi T, Hatanaka M
Department of Serology and Immunology, Kyoto University.
Biol Chem Hoppe Seyler. 1988 May;369 Suppl:223-7.
Thirteen cell lines of the human hematopoietic system were tested for expression of calpastatin at three different levels (mRNA, product and activity). The amount of calpastatin product and its inhibitory activity varied markedly depending upon cell types or human T-cell leukemia virus type I infection, although there were difference in the expression at the transcriptional level. Cell lines with high content of calpastatin comprised T-cell lineage infected by human T-cell leukemia virus type I, B-cell line and myelocytic leukemia cell lines. Non-infected T-cell lines and null cell line revealed themselves as poor in both calpastatin products and inhibitor activities. Two different molecular forms of calpastatin were observed: a 102-kDa form in T- and B-cell lines and a 94-kDa form in one of myelocytic cells. Three mRNA species of calpastatin (3.8, 3.0 and 2.5 kb) were identified in human hematopoietic system cells.
对13种人类造血系统细胞系进行了检测，以分析钙蛋白酶抑制蛋白在三个不同水平（mRNA、产物和活性）的表达情况。尽管转录水平的表达存在差异，但钙蛋白酶抑制蛋白产物的量及其抑制活性因细胞类型或I型人类T细胞白血病病毒感染而有显著差异。钙蛋白酶抑制蛋白含量高的细胞系包括受I型人类T细胞白血病病毒感染的T细胞系、B细胞系和髓细胞白血病细胞系。未感染的T细胞系和裸细胞系在钙蛋白酶抑制蛋白产物和抑制活性方面均表现较差。观察到两种不同分子形式的钙蛋白酶抑制蛋白：T细胞系和B细胞系中为102 kDa的形式，一种髓细胞中为94 kDa的形式。在人类造血系统细胞中鉴定出三种钙蛋白酶抑制蛋白的mRNA种类（3.8、3.0和2.5 kb）。
