Wdowiak Artur, Raczkiewicz Dorota, Stasiak Magdalena, Bojar Iwona
Laboratory of Diagnostic Procedures, Faculty of Nursing and Health Sciences, Medical University, Lublin, Poland.
Institute of Statistics and Demography, Warsaw School of Economics, Warsaw, Poland.
Neuro Endocrinol Lett. 2014;35(1):73-9.
Having an offspring is the most important human biological goal, which is necessary for survival of the human species. Lack of offspring is a phenomenon concerning approximately 15% of married couples in Poland. In a half of the cases, a causative agent is the male factor infertility problem. There is evidence that certain male fertility problems are related with disorders of the process of spermatogenesis. The course of normal spermatogenesis depends on proper pituitary secretion of folliculostimulin (FSH), luteinizing hormone (LH), as well as testicular secretion of testosterone. It is considered that in approximately 20% of patients with idiopathic infertility an elevated level of sperm DNA fragmentation may be the cause of failure in reproduction. The objective of the present study was determination of the relationship between FSH, LH and testosterone levels, and the occurrence of sperm DNA fragmentation.
The present study was conducted in the year 2012 in the Non-Public Health Care Unit 'Ovum Reproduction and Andrology' in Lublin, and covered 186 men treated for infertility. For inclusion into the study group we qualified males aged 25-35, who have been treated for infertility for more than 1 year, with no pathological features observed in the female partner. The structure of sperm chromatin was evaluated using the technique of flow cytometry-Sperm Chromatin Structure assay (SCSA). The result of the examination was a sperm DNA Fragmentation Index (DFI), i.e., the percentage of sperm with DNA lesions (DNA fragmentation). A morning blood sample (5 mL volume) was obtained and sent to an authorized laboratory to assess serum levels of testosterone, LH and FSH.
An intensified sperm DNA fragmentation co-occurred with both extremely low and extremely high levels of FSH and LH. Sperm DNA fragmentation was negatively correlated with testosterone level.
生育后代是人类最重要的生物学目标，对人类物种的生存至关重要。在波兰，约15%的已婚夫妇存在生育问题。其中，一半情况是由男性因素导致的不育问题。有证据表明，某些男性生育问题与精子发生过程紊乱有关。正常精子发生过程取决于垂体促卵泡素（FSH）、黄体生成素（LH）的正常分泌，以及睾丸睾酮的分泌。据认为，在约20%的特发性不育患者中，精子DNA碎片化水平升高可能是生殖失败的原因。本研究的目的是确定FSH、LH和睾酮水平与精子DNA碎片化发生之间的关系。
本研究于2012年在卢布林的非公立医疗单位“卵子生殖与男科学”进行，涵盖186名接受不育治疗的男性。纳入研究组的男性年龄在25至35岁之间，因不育接受治疗超过1年，其女性伴侣无病理特征。使用流式细胞术-精子染色质结构分析（SCSA）技术评估精子染色质结构。检查结果为精子DNA碎片化指数（DFI），即有DNA损伤（DNA碎片化）的精子百分比。采集一份清晨血样（5毫升），送至授权实验室评估血清睾酮、LH和FSH水平。
精子DNA碎片化加剧与FSH和LH的极低水平及极高水平同时出现。精子DNA碎片化与睾酮水平呈负相关。
