Reddy Mettu S, Carter Noel, Cunningham Anne, Shaw James, Talbot David
School of Applied Sciences, University of Sunderland, Sunderland, UK.
Transpl Int. 2014 Jun;27(6):634-9. doi: 10.1111/tri.12313. Epub 2014 Apr 12.
Success of clinical pancreatic islet transplantation depends on the mass of viable islets transplanted and the proportion of transplanted islets that survive early ischaemia reperfusion injury. Novel pancreas preservation techniques to improve islet preservation and viability can increase the utilization of donation after cardiac death donor pancreases for islet transplantation. Rat pancreases were retrieved after 30 min of warm ischaemia and preserved by static cold storage, hypothermic machine perfusion or retrograde portal venous oxygen persufflation for 6 h. They underwent collagenase digestion and density gradient separation to isolate islets. The yield, viability, morphology were compared. In vitro function of isolated islets was compared using glucose stimulated insulin secretion test. Portal venous oxygen persufflation improved the islet yield, viability and morphology as compared to static cold storage. The percentage of pancreases with good in vitro function (stimulation index > 1.0) was also higher after oxygen persufflation as compared to static cold storage. Retrograde portal venous oxygen persufflation of donation after cardiac death donor rat pancreases has the potential to improve islet yield.
临床胰岛移植的成功取决于移植的有活力胰岛的数量以及移植胰岛在早期缺血再灌注损伤中存活的比例。改善胰岛保存和活力的新型胰腺保存技术可提高心脏死亡供体胰腺用于胰岛移植的利用率。大鼠胰腺在热缺血30分钟后取出，通过静态冷藏、低温机器灌注或逆行门静脉氧吹入法保存6小时。然后进行胶原酶消化和密度梯度分离以分离胰岛。比较了胰岛的产量、活力和形态。使用葡萄糖刺激胰岛素分泌试验比较了分离胰岛的体外功能。与静态冷藏相比，逆行门静脉氧吹入法提高了胰岛产量、活力和形态。与静态冷藏相比，氧吹入法后体外功能良好（刺激指数>1.0）的胰腺百分比也更高。心脏死亡供体大鼠胰腺的逆行门静脉氧吹入法有可能提高胰岛产量。
