导致妊娠期 T 调节细胞定位的母体和胎儿因素。
Maternal and fetal factors that contribute to the localization of T regulatory cells during pregnancy.
机构信息
Division of Reproductive Endocrinology and Infertility, Department of OB/GYN, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
出版信息
Am J Reprod Immunol. 2014 May;71(5):391-400. doi: 10.1111/aji.12223. Epub 2014 Mar 14.
PROBLEM
To determine the interplay between fetal antigenicity and local maternal factors in determining reproductive tract T regulatory (Treg) cell accumulation during pregnancy.
METHOD OF STUDY
Examination of maternal Treg composition in the uterus, cervix, and uteroplacental interface (UPI) of murine syngeneic and allogeneic pregnancies and non-pregnant controls by flow cytometry. The impact of fetal antigenicity was defined by either fetal gender in syngeneic pregnancies or by allogeneic paternity. Impact of IL-6 on local Treg composition was determined using syngeneic pregnancies in IL-6(-/-) females.
RESULTS
An increased fraction of CD4(+) T cells in the pregnant uterine lymphocytic infiltrate and draining pelvic lymph nodes are Tregs. Maternal IL-6 decreases Treg accumulation within the uterus and to a greater extent in the cervix in syngeneic pregnancy. Fetal antigenicity is matched by accumulation of Tregs to the UPI. Treg accumulation at the UPI of non-antigenic female fetuses is determined by the intrauterine position relative to male siblings.
CONCLUSION
Reproductive tract tissue Treg composition during pregnancy is influenced by maternal IL-6 and fetal antigenicity.
问题
确定胎儿抗原性与局部母体因素在妊娠期间生殖道调节性 T 细胞（Treg）积累中的相互作用。
研究方法
通过流式细胞术检查鼠同基因和同种异体妊娠以及非妊娠对照的子宫、宫颈和胎盘界面（UPI）中的母体 Treg 组成。通过同基因妊娠中的胎儿性别或同种异体父系来定义胎儿抗原性的影响。使用 IL-6（-/-）雌性的同基因妊娠来确定 IL-6 对局部 Treg 组成的影响。
结果
妊娠子宫淋巴细胞浸润和引流盆腔淋巴结中的 CD4(+)T 细胞的比例增加是 Treg。母性 IL-6 减少了同基因妊娠中子宫内和宫颈内 Treg 的积累。胎儿抗原性与 UPI 处 Treg 的积累相匹配。非抗原性雌性胎儿 UPI 处 Treg 的积累取决于相对于雄性同胞的子宫内位置。
结论
妊娠期间生殖道组织 Treg 组成受母体 IL-6 和胎儿抗原性的影响。
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