Ho Kevin K Y, Leung Priscilla T Y, Ip Jack C H, Qiu J W, Leung Kenneth M Y
The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.
Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong, China; State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
Mar Pollut Bull. 2014 Aug 30;85(2):499-504. doi: 10.1016/j.marpolbul.2014.02.023. Epub 2014 Mar 12.
The intertidal whelk Reishia clavigera (formerly named as Thais clavigera) is one of the most sensitive species to organotin-associated imposex. However, the limited information on mRNA transcriptome of the species has restricted the molecular investigation on such endocrine disruption. By means of Illumina sequencing, we obtained a global de novo transcriptome from the gonadal tissues of both male and female R. clavigera, with 197,324 assembled transcripts and 151,684 condensed non-redundant transcripts. Blast hit results from the NCBI's non-redundant molluscan database showed that 28,948 transcripts were successfully annotated with significant matches at an e-value of ⩽1e(-6). Among them, 1108 transcripts were assigned a well-defined gene ontology term. As the first transcriptomic study on the gonadal tissues of R. clavigera, this study has enhanced the information of mRNA transcriptome on this species and will thus facilitate mechanistic studies of chemical contaminants (e.g., organotins) on this common biomonitor species.
潮间带蛾螺Reishia clavigera（以前名为Thais clavigera）是对有机锡相关性畸变最敏感的物种之一。然而，关于该物种mRNA转录组的信息有限，限制了对这种内分泌干扰的分子研究。通过Illumina测序，我们从雌雄R. clavigera的性腺组织中获得了一个全局从头转录组，有197,324个组装转录本和151,684个浓缩的非冗余转录本。来自NCBI非冗余软体动物数据库的Blast比对结果显示，28,948个转录本在e值⩽1e(-6)时成功注释并具有显著匹配。其中，1108个转录本被赋予了明确的基因本体术语。作为对R. clavigera性腺组织的首次转录组学研究，本研究增加了该物种mRNA转录组的信息，从而将促进对这种常见生物监测物种的化学污染物（如有机锡）的作用机制研究。
