Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China.
Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Food Chem. 2014 Aug 1;156:64-71. doi: 10.1016/j.foodchem.2014.01.096. Epub 2014 Feb 5.
Alkali-soluble pectin, which has been extracted from Canna edulis Ker, was characterized by single sugar determination and atomic force microscopy (AFM). The results indicated that the amounts of four predominant sugars including arabinose (Ara), glucose (Glc), galactose (Gal) and galacturonic acid (GalA) significantly decreased during the process of mild acid hydrolysis. The decreasing rates of these four sugars followed a sequential order of Ara>Gal>Glc>GalA. The homogalacturonan (HG) chain present in pectin, and the quantity of branched material is greater than the sample containing the main neutral sugars. The results indicated that the neutral sugar and HG side chains are attached to pectin as part of the rhamnogalacturonan I (RGI) complex. Moreover, hydrolysis leads to the reduction of mean lengths of backbone and branch, as well as the number/weight-average molecular weight. Meanwhile, the amount of short chain fractions increased during hydrolysis. Furthermore, the decrease of the polymerization degree of alkali-soluble C. edulis pectin as a function of the hydrolysis time could be described by a first-order exponential decay function.
从美人蕉中提取的碱溶性果胶，通过单糖测定和原子力显微镜（AFM）进行了表征。结果表明，在温和酸水解过程中，四种主要糖（包括阿拉伯糖（Ara）、葡萄糖（Glc）、半乳糖（Gal）和半乳糖醛酸（GalA））的含量显著下降。这四种糖的减少率遵循 Ara>Gal>Glc>GalA 的顺序。果胶中存在的同质半乳糖醛酸（HG）链和支化物质的数量大于主要中性糖的样品。结果表明，中性糖和 HG 侧链作为鼠李半乳糖醛酸 I（RGI）复合物的一部分连接到果胶上。此外，水解导致主链和支链的平均长度以及数均/重均分子量降低。同时，在水解过程中短链分数的量增加。此外，碱溶性美人蕉果胶的聚合度随水解时间的减少可以用一级指数衰减函数来描述。
