Department of Life Sciences, University of Limerick, Limerick, Ireland; Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE, Università di Padova, Legnaro, Padova, Italy.
Department of Life Sciences, University of Limerick, Limerick, Ireland.
Food Chem. 2014 Aug 1;156:305-11. doi: 10.1016/j.foodchem.2014.01.080. Epub 2014 Feb 5.
A Ficus carica L. latex proteinase preparation was investigated for its ability to produce antioxidant hydrolysates/peptides from bovine casein (CN). The Oxygen Radical Absorbance Capacity (ORAC) values for NaCN and β-CN hydrolysates ranged from 0.06 to 0.18, and from 0.51 to 1.19μmol Trolox equivalents/mg freeze-dried sample, respectively. Gel permeation HPLC showed that the β-CN hydrolysate with a degree of hydrolysis of 21% had 65% of peptide material with a molecular mass <500Da. The RP-UPLC profiles also indicated that β-CN was substantially hydrolysed during the early stages of hydrolysis. Analysis of the 4h β-CN hydrolysate by LC-ESI-MS/MS allowed identification of 8 peptide sequences with potential antioxidant properties.
研究了一种榕树叶乳蛋白酶制剂从牛酪蛋白（CN）生产抗氧化水解物/肽的能力。NaCN 和 β-CN 水解产物的氧自由基吸收能力（ORAC）值分别为 0.06 至 0.18 和 0.51 至 1.19μmol Trolox 当量/毫克冻干样品。凝胶渗透 HPLC 显示，水解度为 21%的β-CN 水解产物中有 65%的肽物质分子量<500Da。RP-UPLC 图谱也表明，β-CN 在水解的早期阶段被大量水解。通过 LC-ESI-MS/MS 对 4 小时β-CN 水解产物进行分析，鉴定出 8 种具有潜在抗氧化特性的肽序列。
