Department of Biological Science, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea; Global Environmental Regulation and Compliance Center, Korea Institute of Toxicology, JinJu 660-844, Republic of Korea.
Global Environmental Regulation and Compliance Center, Korea Institute of Toxicology, JinJu 660-844, Republic of Korea.
Chemosphere. 2014 Aug;108:376-82. doi: 10.1016/j.chemosphere.2014.02.025. Epub 2014 Mar 12.
The objective of this study was to evaluate the ecological health of an urban stream using Integrated Health Responses (IHRs). Water chemistry analysis, habitat health, and ecotoxicity tests were conducted in the stream along with analyses of molecular/biochemical, physiological biomarkers, and population-level responses in indicator species. Chemical stresses, measured as nutrient levels, ionic content and organic matter concentrations were significantly greater (p<0.01) at the downstream than the reference site (RF). The habitat health was largely impacted in the downstream reaches and had a negative relation with the land-use pattern of % urban area. Comet assay, 7-ethoxyresorufin-O-deethylase (EROD), acetylcholinesterase (AChE), and vitellogenin (VTG) were evaluated for low-level biomarker responses on DNA/physiological conditions of target species. The multi-metric fish model (Mm-F) was used to test the community-level response in relation to chemical and physical habitat stresses. The impaired responses of separate biomarker and bioindicator at the downstream sites occurred at all organizations from molecular/biochemical level to community level. Using all biomarkers/bioindicators, the star-plot model of IHRs was developed and then the integrative health/risk assessments were conducted in the urban stream. The reduced values of IHRs occurred in the downstream sites and the impacts were attributed to effluents from wastewater treatment plants (WTPs) and industrial complex. Ecological health impairments, thus, were evident in the urban reach, and reflected the long-term community responses as well as short-term responses of molecular biomarkers. The degradation of the urban stream was mainly due to a combined effect of chemical pollution and physical habitat modifications.
本研究旨在利用综合健康响应（IHRs）评估城市溪流的生态健康状况。在溪流中进行了水质分析、生境健康和生态毒性测试，以及指示物种的分子/生化、生理生物标志物和种群水平响应分析。化学胁迫，以营养水平、离子含量和有机物浓度来衡量，在下游比参考点（RF）显著更高（p<0.01）。下游的生境健康受到了很大的影响，并与城市面积百分比的土地利用模式呈负相关。彗星试验、7-乙氧基resorufin-O-去乙基酶（EROD）、乙酰胆碱酯酶（AChE）和卵黄蛋白原（VTG）用于评估目标物种的低水平生物标志物响应的 DNA/生理状况。多指标鱼类模型（Mm-F）用于测试与化学和物理生境胁迫相关的群落水平响应。下游地点的各个生物标志物/生物指示剂的受损反应出现在从分子/生化水平到群落水平的所有组织中。使用所有的生物标志物/生物指示剂，开发了 IHRs 的星图模型，然后对城市溪流进行综合健康/风险评估。IHRs 的值在下游地点降低，其影响归因于废水处理厂（WTPs）和工业综合体的废水。因此，城市河段的生态健康受到损害，反映了长期的社区响应以及分子生物标志物的短期响应。城市溪流的退化主要是由于化学污染和物理生境变化的综合影响。
