曲霉菌的肽谷氨酰胺酶-天冬酰胺酶的特定蛋白水解消化分析。
Analysis of specific proteolytic digestion of the peptidoglutaminase-asparaginase of koji molds.
机构信息
Noda Institute for Scientific Research, 399 Noda, Noda, Chiba 278-0037, Japan.
Noda Institute for Scientific Research, 399 Noda, Noda, Chiba 278-0037, Japan.
出版信息
J Biosci Bioeng. 2014 Sep;118(3):253-5. doi: 10.1016/j.jbiosc.2014.02.007. Epub 2014 Mar 11.
PMID:24630615
Abstract
AsGahB, a peptidoglutaminase-asparaginase acting as the main glutaminase in Aspergillus sojae, was previously purified from the cytoplasm of overexpressing strains. Here, we found that specific proteolytic digestion of AsGahB by extracellular proteases of koji molds is similar to that of AsGahA which exists in proteolytic form under solid-state culture.
摘要
AsGahB，一种作用于 Aspergillus sojae 中主要谷氨酰胺酶的肽谷氨酰胺酶-天冬酰胺酶，之前是从过表达菌株的细胞质中纯化得到的。在这里，我们发现曲霉菌的细胞外蛋白酶对 AsGahB 的特异性蛋白水解与在固态培养下以蛋白水解形式存在的 AsGahA 相似。
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