Özdemir Özmert M A, Gözkeser Ersin, Bir Ferda, Yenisey Çiğdem
Department of Pediatrics, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Department of Pediatrics, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Pediatr Neonatol. 2014 Oct;55(5):352-7. doi: 10.1016/j.pedneo.2013.11.004. Epub 2014 Mar 11.
Bronchopulmonary dysplasia (BPD) is a chronic lung disease that causes significant morbidity and mortality in premature infants. Inflammation and oxidative injury play an important role in the pathogenesis of BPD. Resveratrol is an antioxidant and anti-inflammatory agent. In this study, the histopathological and biochemical effects of resveratrol on a hyperoxia-induced lung injury model in newborn rats were investigated.
The experiment was performed on newborn rat pups from the 3(rd) to 13(th) postnatal day and they were randomly divided into four groups: Group 1 (air-exposed + saline, n = 10), Group 2 (air-exposed + resveratrol, n = 11), Group 3 (hyperoxia-exposed + saline, n = 6) and Group 4 (hyperoxia-exposed + resveratrol, n = 7). Resveratrol was administered (30 mg/kg/day) intraperitoneally. The histopathological effects of resveratrol on lung tissue were assessed by alveolar surface area, fibrosis, and smooth muscle actin (SMA) score, and the biochemical effects on lung tissue were assessed by glutathione (GSH), superoxide dismutase (SOD), nitric oxide (NO), tumor necrosis factor-α (TNF-α), and nuclear factor kappa B (NF-κB) levels.
The alveolar surface area, fibrosis, SMA score, and NO levels were found to be significantly higher in Group 3 compared with Group 1 (p < 0.05). In addition, it was found that resveratrol treatment significantly reduced the SMA score and the NO and TNF-α levels, and increased the GSH and SOD levels in the hyperoxia group (p < 0.05).
This experimental study showed that oxidative stress and NO contributed to the pathogenesis of hyperoxia-induced lung injury, and that resveratrol had a preventive effect on hyperoxic lung injury through its anti-inflammatory and antioxidant properties.
支气管肺发育不良（BPD）是一种慢性肺部疾病，可导致早产儿出现显著的发病率和死亡率。炎症和氧化损伤在BPD的发病机制中起重要作用。白藜芦醇是一种抗氧化剂和抗炎剂。在本研究中，研究了白藜芦醇对新生大鼠高氧诱导肺损伤模型的组织病理学和生化影响。
实验在出生后第3天至第13天的新生大鼠幼崽上进行，将它们随机分为四组：第1组（空气暴露+生理盐水，n = 10），第2组（空气暴露+白藜芦醇，n = 11），第3组（高氧暴露+生理盐水，n = 6）和第4组（高氧暴露+白藜芦醇，n = 7）。白藜芦醇通过腹腔注射给药（30 mg/kg/天）。通过肺泡表面积、纤维化和平滑肌肌动蛋白（SMA）评分评估白藜芦醇对肺组织的组织病理学影响，通过谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和核因子κB（NF-κB）水平评估对肺组织的生化影响。
发现第3组的肺泡表面积、纤维化、SMA评分和NO水平显著高于第1组（p < 0.05）。此外，发现白藜芦醇治疗显著降低了高氧组的SMA评分以及NO和TNF-α水平，并提高了GSH和SOD水平（p < 0.05）。
本实验研究表明，氧化应激和NO促成了高氧诱导肺损伤的发病机制，并且白藜芦醇通过其抗炎和抗氧化特性对高氧性肺损伤具有预防作用。
