研究果蝇新陈代谢的方法。
Methods for studying metabolism in Drosophila.
作者信息
Tennessen Jason M, Barry William E, Cox James, Thummel Carl S
机构信息
Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112-5330, USA.
Department of Biochemistry and the Metabolomics Core Research Facility, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
出版信息
Methods. 2014 Jun 15;68(1):105-15. doi: 10.1016/j.ymeth.2014.02.034. Epub 2014 Mar 12.
Abstract
Recent research using Drosophila melanogaster has seen a resurgence in studies of metabolism and physiology. This review focuses on major methods used to conduct this work. These include protocols for dietary interventions, measurements of triglycerides, cholesterol, glucose, trehalose, and glycogen, stains for lipid detection, and the use of gas chromatography-mass spectrometry (GC-MS) to detect major polar metabolites. It is our hope that this will provide a useful framework for both new and current researchers in the field.
摘要
最近利用黑腹果蝇开展的研究使得对新陈代谢和生理学的研究再度兴起。本综述聚焦于开展这项工作所使用的主要方法。这些方法包括饮食干预方案、甘油三酯、胆固醇、葡萄糖、海藻糖和糖原的测量、脂质检测染色，以及使用气相色谱-质谱联用仪（GC-MS）检测主要极性代谢物。我们希望这将为该领域的新老研究人员提供一个有用的框架。
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本文引用的文献
1
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Nat Methods. 2014 Jan;11(1):100-5. doi: 10.1038/nmeth.2731. Epub 2013 Nov 17.
2
Mapping an atlas of tissue-specific Drosophila melanogaster metabolomes by high resolution mass spectrometry.通过高分辨率质谱法绘制果蝇组织特异性代谢组图谱
PLoS One. 2013 Oct 29;8(10):e78066. doi: 10.1371/journal.pone.0078066. eCollection 2013.
3
A novel ion pairing LC/MS metabolomics protocol for study of a variety of biologically relevant polar metabolites.一种新型的离子对 LC/MS 代谢组学方法，用于研究多种具有生物学相关性的极性代谢物。
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Oct 1;936:63-73. doi: 10.1016/j.jchromb.2013.07.027. Epub 2013 Aug 7.
4
Development and characterization of a chemically defined food for Drosophila.开发和鉴定一种用于果蝇的化学限定食物。
PLoS One. 2013 Jul 2;8(7):e67308. doi: 10.1371/journal.pone.0067308. Print 2013.
5
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FEBS Open Bio. 2012 Jul 26;2:217-21. doi: 10.1016/j.fob.2012.07.007. Print 2012.
6
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