Grayson Ben, Adamson Lisa, Harte Michael, Leger Marianne, Marsh Samuel, Piercy Chloe, Neill Joanna C
Manchester Pharmacy School, University of Manchester, Manchester M13 9PT, UK.
Manchester Pharmacy School, University of Manchester, Manchester M13 9PT, UK.
Behav Brain Res. 2014 Jun 1;266:188-92. doi: 10.1016/j.bbr.2014.03.011. Epub 2014 Mar 13.
Recognition memory, impaired in neuropsychiatric conditions and currently untreated, may be assessed by the novel object recognition (NOR) task with robust impairments induced by sub-chronic treatment with the N-methyl-d-aspartate receptor antagonist phencyclidine (PCP). The aim of the present study was to investigate how sub-chronic PCP produces its effects in this task. Forty adult female rats received vehicle or PCP (2mg/kg i.p. twice daily for 7 days followed by 7 days washout). Rats completed a 3-min acquisition trial followed by differential inter-trial-interval (ITI) conditions (1 min in the home cage, 10s in the home cage, 1 min in the NOR test box in the presence of an unfamiliar object or 1 min in the NOR test box completely undisturbed) followed by a 3-min retention trial. Control rats spent significantly more time exploring the novel compared with the familiar object in retention. This effect was abolished in the sub-chronic PCP treated animals following all ITI conditions except in rats left completely undisturbed in the NOR test box for a 1 min ITI. The combined influence of sub-chronic PCP treatment and the effect of distraction provides further support for the validity of the NOR test in mimicking cognitive deficits of relevance to schizophrenia.
在神经精神疾病中受损且目前未接受治疗的识别记忆，可通过新颖物体识别（NOR）任务进行评估，用N-甲基-D-天冬氨酸受体拮抗剂苯环利定（PCP）进行亚慢性治疗会导致该任务出现明显损伤。本研究的目的是探究亚慢性PCP在该任务中是如何产生其作用的。40只成年雌性大鼠接受了溶剂或PCP（2mg/kg腹腔注射，每天两次，持续7天，随后洗脱7天）。大鼠先完成一个3分钟的获取试验，然后是不同的试验间隔（ITI）条件（在家笼中1分钟、在家笼中10秒、在存在陌生物体的NOR试验箱中1分钟或在NOR试验箱中完全不受干扰1分钟），随后是一个3分钟的保持试验。在保持试验中，对照大鼠花在探索新颖物体上的时间明显多于熟悉物体。在亚慢性PCP处理的动物中，除了在NOR试验箱中完全不受干扰1分钟的ITI条件下，所有ITI条件后的这种效应均被消除。亚慢性PCP治疗的综合影响和分心效应进一步支持了NOR试验在模拟与精神分裂症相关的认知缺陷方面的有效性。
