Coruh Ceyda, Shahid Saima, Axtell Michael J
Department of Biology, Penn State University, University Park, PA 16802, USA; Plant Biology Intercollegiate Ph.D. Program, Penn State University, University Park, PA 16802, USA; Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Department of Biology, Penn State University, University Park, PA 16802, USA; Plant Biology Intercollegiate Ph.D. Program, Penn State University, University Park, PA 16802, USA; Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Curr Opin Plant Biol. 2014 Apr;18:87-95. doi: 10.1016/j.pbi.2014.02.008. Epub 2014 Mar 15.
A key goal in genomics is the complete annotation of the expressed regions of the genome. In plants, substantial portions of the genome make regulatory small RNAs produced by Dicer-Like (DCL) proteins and utilized by Argonaute (AGO) proteins. These include miRNAs and various types of endogenous siRNAs. Small RNA-seq, enabled by cheap and fast DNA sequencing, has produced an enormous volume of data on plant miRNA and siRNA expression in recent years. In this review, we discuss recent progress in using small RNA-seq data to produce stable and reliable annotations of miRNA and siRNA genes in plants. In addition, we highlight key goals for the future of small RNA gene annotation in plants.
基因组学的一个关键目标是对基因组的表达区域进行完整注释。在植物中，基因组的很大一部分产生由类Dicer（DCL）蛋白生成并被AGO蛋白利用的调控性小RNA。这些包括miRNA和各种类型的内源性siRNA。近年来，得益于廉价且快速的DNA测序技术，小RNA测序产生了关于植物miRNA和siRNA表达的大量数据。在本综述中，我们讨论了利用小RNA测序数据对植物miRNA和siRNA基因进行稳定可靠注释的最新进展。此外，我们强调了植物小RNA基因注释未来的关键目标。
