Afshari Afsoon, Yaghobi Ramin, Karimi Mohammad Hossein, Darbooie Mojtaba, Azarpira Negar
Department of Molecular Genetics, Science and Research, Islamic Azad University, Fars, Iran.
Iran J Immunol. 2014 Mar;11(1):29-39.
Interleukin-17 (IL-17), as a potent proinflammatory cytokine, has a critical role in post liver transplant outcomes. However, there is not much information about the effects of IL-17 cytokine on acute liver rejection.
To evaluate the role of IL-17 in post-liver transplant acute rejection.
Ninety seven adult liver transplant patients who enrolled in this cross sectional study were divided into Non-Acute Rejected (Non-AR) and Acute Rejected (AR) patient groups. Three blood samples were collected from each patient in days 1, 4 and 7 post liver transplantation. The IL-17 mRNA levels were evaluated using an in-house real time PCR protocol. IL-17 protein levels were also analyzed in Non-AR, AR and also control groups using ELISA method.
The IL-17 mRNA expression level continuously increased in AR patients in all days of follow-up post liver transplantation. IL-17 expression was, however, down regulated after day 4 post-transplant follow-up in Non-AR patients. Both IL-17 mRNA expression and protein levels were also significantly increased in AR patients compared with Non-AR ones.
Based on these findings, significant increase of IL-17 mRNA and protein levels in AR patients highlights the important role of IL-17 in acute liver rejection.
白细胞介素-17（IL-17）作为一种强效促炎细胞因子，在肝移植术后结局中起关键作用。然而，关于IL-17细胞因子对急性肝排斥反应的影响，目前所知甚少。
评估IL-17在肝移植术后急性排斥反应中的作用。
纳入本横断面研究的97例成年肝移植患者被分为非急性排斥（Non-AR）组和急性排斥（AR）组。在肝移植术后第1、4和7天，从每位患者采集三份血样。使用内部实时PCR方案评估IL-17 mRNA水平。还采用ELISA法分析了Non-AR组、AR组及对照组的IL-17蛋白水平。
肝移植术后随访的所有时间里，AR患者的IL-17 mRNA表达水平持续升高。然而，Non-AR患者在移植后第4天的随访中，IL-17表达下调。与Non-AR患者相比，AR患者的IL-17 mRNA表达和蛋白水平也显著升高。
基于这些发现，AR患者中IL-17 mRNA和蛋白水平的显著升高突出了IL-17在急性肝排斥反应中的重要作用。
