Measuring microtubule growth and gliding in Caenorhabditis elegans embryos.

作者信息

Tegha-Dunghu Justus, Gusnowski Eva M, Srayko Martin

机构信息

Department of Biological Sciences, University of Alberta, CW405, Biological Sciences Building, Edmonton, Canada, AB T6G 2E9.

出版信息

Methods Mol Biol. 2014;1136:103-16. doi: 10.1007/978-1-4939-0329-0_7.

DOI:10.1007/978-1-4939-0329-0_7

PMID:24633796

Abstract

Microtubule plus-tip tracking is a powerful method to measure microtubule growth dynamics in vivo. Here we outline an approach that exploits live confocal microscopy of a GFP-tagged EB1-like protein to measure microtubule growth behavior and minus-end-directed microtubule motor activity at the cortex of Caenorhabditis elegans embryos. The EB1 velocity assay (EVA) provides a method to reproducibly monitor motor- and non-motor-assisted microtubule movements.

摘要