Karakaş Metin O, Türktaş M, Ertuğrul F, Kaya E
TUBITAK Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey
Department of Biology, Faculty of Science, Cankırı Karatekin University, Cankiri, Turkey.
Genet Mol Res. 2014 Mar 6;13(1):1480-90. doi: 10.4238/2014.March.6.6.
The study of phylogenetic relationships between 14 Colchicum taxa spread throughout Turkey was performed using a fluorescent-based amplified fragment length polymorphism (AFLP) technique. Five primer pair combinations were used in AFLP reactions. The data set was analyzed statistically using the NTSYS 2.1 software, and the neighbor-joining and maximum parsimony methods were implemented to generate phylogenetic trees. These analyses clustered the samples into 3 main clades. Both the neighbor-joining and maximum parsimony analyses resulted in similar topologies. Furthermore, supporting the phylogenetic trees, a similar grouping of 14 taxa was generated by principal component analysis. The AFLP analysis with 5 primer combinations was carried out to assess 14 taxa. Fragment sizes ranged from 54 to 462 bp in length for each primer combination. The average was 166 fragments per primer pair, primer B2 generated the highest number of bands (200), and primer B3 produced the lowest number of bands (112). A total of 834 polymorphic bands were scored. The cophenetic correlation coefficient between the data matrix and the cophenetic matrix for AFLP data was 0.72. Based on this molecular data, we concluded that the genetic diversity among these Turkish accessions is relatively high.
利用基于荧光的扩增片段长度多态性（AFLP）技术，对分布于土耳其各地的14个秋水仙属分类群之间的系统发育关系进行了研究。在AFLP反应中使用了5对引物组合。利用NTSYS 2.1软件对数据集进行统计分析，并采用邻接法和最大简约法构建系统发育树。这些分析将样本聚类为3个主要分支。邻接法和最大简约法分析得到的拓扑结构相似。此外，主成分分析也得到了14个分类群的相似分组，支持了系统发育树。采用5种引物组合进行AFLP分析以评估14个分类群。每种引物组合的片段大小在54至462 bp之间。平均每个引物对有166个片段，引物B2产生的条带数最多（200条），引物B3产生的条带数最少（112条）。共记录了834条多态性条带。AFLP数据的数据矩阵与协表型矩阵之间的协表型相关系数为0.72。基于这些分子数据，我们得出结论，这些土耳其种质之间的遗传多样性相对较高。
