Qian X, Gong M J, Wang C X, Tian M
Research Institution of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang Province, China.
Research Institution of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang Province, China
Genet Mol Res. 2014 Feb 21;13(3):6303-15. doi: 10.4238/2014.February.21.5.
The flower developmental process, which is crucial to the whole lifecycle of higher plants, is influenced by both environmental and endogenous factors. The genus Oncidium is commercially important for cut flower and houseplant industry and is ideal for flower development studies. Using cDNA-amplified restriction fragment length polymorphism analysis, we profiled transcripts that are differentially expressed during flower development of Oncidium Milliongolds. A total of 15,960 transcript-derived fragments were generated, with 114 primer sets. Of these, 1248 were sequenced, producing 993 readable sequences. BLASTX/N analysis showed that 833 of the 993 transcripts showed homology to genes in the NCBI databases, exhibiting functions involved in various processes, such as signal transduction, energy conversion, metabolism, and gene expression regulation. The full-length mRNAs of SUCROSE SYNTHASE 1 (SUS1) and LEAFY (LFY) were cloned, and their expression patterns were characterized. The results showed that the expression levels of SUS1 and LFY were similar during flower development. To confirm the function of SUS1 in flower buds, carbohydrate content and sucrose synthase activity were determined. The results showed that changes in sucrose content and sucrose synthase activity reflected SUS1 expression levels. Collectively, these results indicate that SUS1 influences flower development by regulating LFY expression levels through changing the sucrose content of flower buds.
花的发育过程对高等植物的整个生命周期至关重要，它受到环境和内源因素的影响。文心兰属对于切花和室内盆栽产业具有重要的商业价值，是花发育研究的理想材料。利用cDNA扩增片段长度多态性分析，我们对文心兰“百万金”花发育过程中差异表达的转录本进行了分析。用114对引物共产生了15960个转录本衍生片段。其中，对1248个进行了测序，得到993个可读序列。BLASTX/N分析表明，993个转录本中的833个与NCBI数据库中的基因具有同源性，其功能涉及信号转导、能量转换、代谢和基因表达调控等多个过程。克隆了蔗糖合成酶1（SUS1）和叶状（LFY）的全长mRNA，并对其表达模式进行了表征。结果表明，SUS1和LFY在花发育过程中的表达水平相似。为了证实SUS1在花芽中的功能，测定了碳水化合物含量和蔗糖合成酶活性。结果表明，蔗糖含量和蔗糖合成酶活性的变化反映了SUS1的表达水平。总的来说，这些结果表明SUS1通过改变花芽中的蔗糖含量来调节LFY的表达水平，从而影响花的发育。
