Miller-Patrick K, Ballough G P, Wickersham E W, Anthony A
Biology Department, Pennsylvania State University, University Park 16802.
Life Sci. 1988;43(26):2231-9. doi: 10.1016/0024-3205(88)90416-x.
Female Sprague-Dawley rats (160-180g.) with normal estrous cyclicity established by vaginal smears, were injected intraperitoneally with 0.45 mg/kg (low dose) or 0.68 mg/kg (high dose) of T-2 toxin, a trichothecene with potent protein inhibitory abilities. Control animals were injected with only the 100% ethanol vehicle. All animals were decapitated at 8 hours post-exposure, and their ovaries removed and processed for paraffin sectioning. Coomassie, Feulgen, and azure B/DNase staining procedures were used to show granulosa cell protein levels, F-DNA stainability, and basophilia/RNA levels, respectively. Quantification of these parameters was accomplished using scanning-integrating microdensitometry. T-2 toxin treatment groups had granulosa cell protein levels significantly lower than those of the control animals. However, rats exposed to the lower dose of T-2 toxin generally showed a more marked suppression of protein levels than the high dose group, regardless of the stage of the estrous cycle. In addition, rats that received lower doses of T-2 toxin had impaired translation and template activity in response to injury, when compared with the rats in the high dose group. These results are attributed to the lesser degree of circulatory impairment in the low T-2 toxin dosage group, which allows a higher amount of T-2 toxin to interact with the cells.
通过阴道涂片确定具有正常发情周期的雌性斯普拉格-道利大鼠（160 - 180克），腹腔注射0.45毫克/千克（低剂量）或0.68毫克/千克（高剂量）的T-2毒素，这是一种具有强大蛋白质抑制能力的单端孢霉烯族毒素。对照动物仅注射100%乙醇赋形剂。所有动物在暴露后8小时断头，取出卵巢并进行石蜡切片处理。分别使用考马斯亮蓝、福尔根和天青B/脱氧核糖核酸酶染色程序来显示颗粒细胞蛋白质水平、F-脱氧核糖核酸染色性和嗜碱性/核糖核酸水平。使用扫描积分显微密度测定法对这些参数进行定量。T-2毒素处理组的颗粒细胞蛋白质水平显著低于对照动物。然而，无论发情周期处于哪个阶段，接触低剂量T-2毒素的大鼠通常比高剂量组表现出更明显的蛋白质水平抑制。此外，与高剂量组大鼠相比，接受低剂量T-2毒素的大鼠在受伤时翻译和模板活性受损。这些结果归因于低T-2毒素剂量组循环损伤程度较小，这使得更多的T-2毒素能够与细胞相互作用。
