基于感觉刺激的完全性缺血性卒中保护在大脑中动脉闭塞后4个月仍保持稳定。

Sensory Stimulation-Based Complete Protection from Ischemic Stroke Remains Stable at 4 Months Post-Occlusion of MCA.

作者信息

Hancock Aneeka M, Lay Christopher C, Davis Melissa F, Frostig Ron D

机构信息

Department of Neurobiology and Behavior, University of California, Irvine, California, USA ; The Center for the Neurobiology of Learning and Memory, University of California, Irvine, California, USA.

Department of Neurobiology and Behavior, University of California, Irvine, California, USA ; The Center for the Neurobiology of Learning and Memory, University of California, Irvine, California, USA ; The Center for Hearing Research, University of California, Irvine, California, USA.

出版信息

J Neurol Disord. 2013 Nov;1(4):135. doi: 10.4172/2329-6895.1000135.

Previous research from our lab has shown that when using a rodent model of ischemic stroke (permanent middle cerebral artery occlusion), mild sensory stimulation, when delivered within two hours of ischemic onset, completely protects the cortex from impending ischemic stroke damage when assessed 24 hours post-occlusion. However, the long-term stability of this protection remains unclear. Using intrinsic signal optical imaging for assessment of cortical function, laser speckle imaging for assessment of blood flow, a battery of behavioral tests and cresyl violet for histological assessment, the present study examined whether this protection was long-lasting. When assessed 4 months post-occlusion (this length of time being equivalent to 10-15 years in humans), rats receiving sensory stimulation treatment immediately after ischemic onset exhibit normal neuronal and vascular function, and they are behaviorally and histologically equivalent to healthy controls (surgical shams). Thus, the complete neuroprotection due to cortical activation via sensory stimulation remains stable with time. These findings add support to the translational potential of this sensory stimulation-based treatment.

我们实验室之前的研究表明，在使用缺血性中风的啮齿动物模型（永久性大脑中动脉闭塞）时，轻度感觉刺激在缺血发作后两小时内给予，在闭塞后24小时评估时，能完全保护皮质免受即将发生的缺血性中风损伤。然而，这种保护的长期稳定性尚不清楚。本研究使用内在信号光学成像评估皮质功能、激光散斑成像评估血流、一系列行为测试以及用甲酚紫进行组织学评估，来检验这种保护是否持久。在闭塞后4个月进行评估时（这段时间相当于人类的10 - 15年），缺血发作后立即接受感觉刺激治疗的大鼠表现出正常的神经元和血管功能，并且在行为和组织学上与健康对照（假手术组）相当。因此，通过感觉刺激激活皮质所产生的完全神经保护作用会随时间保持稳定。这些发现为这种基于感觉刺激的治疗的转化潜力提供了支持。