Queiroz Júnior Luiz de Pádua, de Camargo Zoilo Pires, Tadano Tomoko, Rodrigues Anderson Messias, Takarara Doracilde Terumi, Gegembauer Gregory, Araujo Leticia Mendes, Hahn Rosane Christine
Mycology/Research Laboratory, Faculty of Medicine, Federal University of Mato Grosso, UFMT, Cuiabá, Mato Grosso, Brazil.
Mycoses. 2014 Aug;57(8):466-72. doi: 10.1111/myc.12183. Epub 2014 Mar 18.
Clinical Paracoccidioides spp. isolates from patients with paracoccidioidomycosis (PCM) in Mato Grosso, Brazil exhibit different patterns of serologic reactivity. The results observed for reactions of radial immunodiffusion against the commonly used exoantigens containing a 43-kDa glycoprotein (gp43) suggest that this fungus exhibits major antigenic variability by geographic region. There is a phylogenetic gap between Paracoccidioides spp. isolates among different regions of Latin America. In particular, those from the central region of Brazil (i.e. Mato Grosso state) exhibit a lower rate of genetic similarity. We aimed at investigating the phylogenetic classification of clinical isolates of Paracoccidioides spp. in Central Brazil and the different antigenic profiles that produce. Exoantigens were obtained from five clinical isolates: two P. brasiliensis (Pb166 and Pb2880) and three P. lutzii (PL2875, PL9840, and PL2912). The protein/glycoprotein profiles of P. lutzii exoantigens were different from each other. Isolate PL9840 exhibited the most distinct bands, and isolates PL2875 and PL2912 exhibited more diffuse bands and a very intense band between 50 and 60 kDa. P. brasiliensis isolates had similar protein profiles, exhibiting a low-intensity band at 220 kDa and a diffuse band between 50 and 60 kDa. P. lutzii isolates exhibit high species-specific antigen variability, which we have already been assessed in proteomic studies.
来自巴西马托格罗索州副球孢子菌病（PCM）患者的临床副球孢子菌属分离株表现出不同的血清学反应模式。针对常用的含43 kDa糖蛋白（gp43）外抗原的径向免疫扩散反应结果表明，这种真菌在不同地理区域表现出主要的抗原变异性。拉丁美洲不同地区的副球孢子菌属分离株之间存在系统发育差距。特别是来自巴西中部地区（即马托格罗索州）的分离株表现出较低的遗传相似率。我们旨在研究巴西中部副球孢子菌属临床分离株的系统发育分类及其产生的不同抗原谱。从五株临床分离株中获得外抗原：两株巴西副球孢子菌（Pb166和Pb2880）和三株卢氏副球孢子菌（PL2875、PL9840和PL2912）。卢氏副球孢子菌外抗原的蛋白质/糖蛋白谱彼此不同。分离株PL9840表现出最明显的条带，分离株PL2875和PL2912表现出更多的弥散条带以及一条位于50至60 kDa之间的非常强的条带。巴西副球孢子菌分离株具有相似的蛋白质谱，在220 kDa处有一条低强度条带，在50至60 kDa之间有一条弥散条带。卢氏副球孢子菌分离株表现出高度物种特异性的抗原变异性，我们已经在蛋白质组学研究中对其进行了评估。
