1 型糖尿病患儿自身免疫性肝炎相关自身抗体。

Autoimmune hepatitis related autoantibodies in children with type 1 diabetes.

机构信息

Department of Pediatrics at King Fahad Medical City, Riyadh, Saudi Arabia.

出版信息

Diabetol Metab Syndr. 2014 Mar 17;6(1):38. doi: 10.1186/1758-5996-6-38.

DOI:10.1186/1758-5996-6-38

PMID:24636465

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3995446/

Abstract

BACKGROUND AND OBJECTIVES

The frequency of Type 1 diabetes (T1D)-related autoantibodies was determined in children with autoimmune hepatitis. However, the incidence of autoimmune hepatitis related autoantibodies in children with T1D has been poorly investigated. The aim of the present cross sectional prospective study was to determine the occurrence of autoimmune hepatitis-related autoantibodies in children with T1D.

METHODS

Children with T1D following in diabetic clinic in our center were screened for existence of liver related autoantibodies from November 2010 to November 2011. The patients' sera were analyzed for the existence of autoantibodies such as anti-nuclear antibody, anti-smooth muscle antibody, and anti-Liver Kidney microsomal antibody, using enzyme linked immunoassay and indirect immunofluorescence methods. A titer of anti-nuclear antibody ≥1/40 was considered positive and titer of < 1/40 was considered negative. Anti-liver kidney microsomal antibody titer of < 3 U/ml was considered negative, 3 - 5 U/ml borderlines, and > 5 U/ml was considered positive.

RESULTS

106 children with T1D have been examined over a one-year period: age ranges between 8 months to 15.5 years, sixty two patients were females. Autoantibody screen revealed a girl with positive anti-liver kidney microsomal antibody (1%) and 8 children had positive anti-nuclear antibody (7.5%), without clinical, biochemical or radiologic evidence of liver disease. None of the patients had positive smooth muscle antibody.

IN CONCLUSION

Anti-liver kidney microsomal antibody is rarely found in sera of children with T1D; the clinical significance of which is unknown.

摘要

背景和目的

本研究旨在确定儿童自身免疫性肝炎中 1 型糖尿病（T1D）相关自身抗体的频率。然而，T1D 患儿自身免疫性肝炎相关自身抗体的发生率尚未得到充分研究。本横断面前瞻性研究的目的是确定 T1D 患儿中是否存在自身免疫性肝炎相关自身抗体。

方法

我们中心的糖尿病门诊对 2010 年 11 月至 2011 年 11 月期间的 T1D 患儿进行了肝相关自身抗体筛查。采用酶联免疫吸附试验和间接免疫荧光法分析患者血清中是否存在抗核抗体、抗平滑肌抗体和抗肝肾微粒体抗体等自身抗体。抗核抗体滴度≥1/40 为阳性，<1/40 为阴性。抗肝肾微粒体抗体滴度<3 U/ml 为阴性，3-5 U/ml 为边界值，>5 U/ml 为阳性。