Department of Pediatrics at King Fahad Medical City, Riyadh, Saudi Arabia.
Diabetol Metab Syndr. 2014 Mar 17;6(1):38. doi: 10.1186/1758-5996-6-38.
The frequency of Type 1 diabetes (T1D)-related autoantibodies was determined in children with autoimmune hepatitis. However, the incidence of autoimmune hepatitis related autoantibodies in children with T1D has been poorly investigated. The aim of the present cross sectional prospective study was to determine the occurrence of autoimmune hepatitis-related autoantibodies in children with T1D.
Children with T1D following in diabetic clinic in our center were screened for existence of liver related autoantibodies from November 2010 to November 2011. The patients' sera were analyzed for the existence of autoantibodies such as anti-nuclear antibody, anti-smooth muscle antibody, and anti-Liver Kidney microsomal antibody, using enzyme linked immunoassay and indirect immunofluorescence methods. A titer of anti-nuclear antibody ≥1/40 was considered positive and titer of < 1/40 was considered negative. Anti-liver kidney microsomal antibody titer of < 3 U/ml was considered negative, 3 - 5 U/ml borderlines, and > 5 U/ml was considered positive.
106 children with T1D have been examined over a one-year period: age ranges between 8 months to 15.5 years, sixty two patients were females. Autoantibody screen revealed a girl with positive anti-liver kidney microsomal antibody (1%) and 8 children had positive anti-nuclear antibody (7.5%), without clinical, biochemical or radiologic evidence of liver disease. None of the patients had positive smooth muscle antibody.
Anti-liver kidney microsomal antibody is rarely found in sera of children with T1D; the clinical significance of which is unknown.
本研究旨在确定儿童自身免疫性肝炎中 1 型糖尿病（T1D）相关自身抗体的频率。然而，T1D 患儿自身免疫性肝炎相关自身抗体的发生率尚未得到充分研究。本横断面前瞻性研究的目的是确定 T1D 患儿中是否存在自身免疫性肝炎相关自身抗体。
我们中心的糖尿病门诊对 2010 年 11 月至 2011 年 11 月期间的 T1D 患儿进行了肝相关自身抗体筛查。采用酶联免疫吸附试验和间接免疫荧光法分析患者血清中是否存在抗核抗体、抗平滑肌抗体和抗肝肾微粒体抗体等自身抗体。抗核抗体滴度≥1/40 为阳性，<1/40 为阴性。抗肝肾微粒体抗体滴度<3 U/ml 为阴性，3-5 U/ml 为边界值，>5 U/ml 为阳性。
在为期一年的时间里，我们对 106 例 T1D 患儿进行了检查：年龄在 8 个月至 15.5 岁之间，其中 62 例为女性。自身抗体筛查显示，一名女孩的抗肝肾微粒体抗体呈阳性（1%），8 名儿童的抗核抗体呈阳性（7.5%），但无肝脏疾病的临床、生化或影像学证据。无一例患者的平滑肌抗体阳性。
T1D 患儿血清中很少发现抗肝肾微粒体抗体；其临床意义尚不清楚。
