Hubbard-Turner Tricia, Guderian Sophie, Turner Michael J
Department of Kinesiology, The University of North Carolina at Charlotte, Charlotte, North Carolina, USA; Department of Mechanical Engineering & Engineering Science, Center for Biomedical Engineering Systems, The University of North Carolina at Charlotte, Charlotte, North Carolina, USA.
Int J Rheum Dis. 2015 Jan;18(1):33-9. doi: 10.1111/1756-185X.12291. Epub 2014 Mar 18.
To examine the effect of lifelong physical activity on the development of knee osteoarthritis (OA).
Fifteen C57Bl/6J mice (8 male and 7 female) were individually caged with a running wheel, magnetic sensor and digital odometer, and 15 mice (7 male and 8 female) were individually housed without a running wheel. Each mouse was provided with standard chow and water ad libitum. Running wheel distance, duration and speed were recorded daily. A diagnostic ultrasound (SONOS 5500 Ultrasound and 15-6L ultrasound probe) was used to measure medial and lateral knee joint space in both hind limbs every month until the end of the mice's lifespans.
Right medial (P = 0.002), right lateral (P < 0.0001), left medial (P = 0.01), and left lateral (P = 0.007) knee joint spaces were significantly larger from months 3 to 12 in the physically active C57Bl/6J mice. However, there was no significant difference between all knee joint spaces of the running and sedentary C57Bl/6J mice throughout the remainder of the lifespan. All hindlimb knee joint spaces significantly declined with aging (P < 0.0001).
During the first 12 months of the lifespan, physical activity seemed to protect the joint from degeneration; however; after that there was no protective benefit. This may be due to the fact that C57Bl/6J mice are genetically known to develop OA at 9 months of age. Physical activity may have no impact on this genetic predisposition. Further study in mice not genetically predisposed to develop OA is needed.
研究终身体育活动对膝关节骨关节炎（OA）发展的影响。
将15只C57Bl/6J小鼠（8只雄性和7只雌性）单独关在配有跑步轮、磁传感器和数字里程表的笼子里，另外15只小鼠（7只雄性和8只雌性）单独饲养，不配备跑步轮。每只小鼠均可自由获取标准食物和水。每天记录跑步轮的距离、持续时间和速度。使用诊断超声（SONOS 5500超声和15 - 6L超声探头）每月测量两只后肢的膝关节内侧和外侧间隙，直至小鼠寿命结束。
在进行体育活动的C57Bl/6J小鼠中，从第3个月到第12个月，右膝内侧（P = 0.002）、右膝外侧（P < 0.0001）、左膝内侧（P = 0.01）和左膝外侧（P = 0.007）的膝关节间隙明显更大。然而，在整个剩余寿命期间，跑步的和久坐不动的C57Bl/6J小鼠的所有膝关节间隙之间没有显著差异。随着年龄增长，所有后肢膝关节间隙均显著减小（P < 0.0001）。
在寿命的前12个月，体育活动似乎能保护关节不发生退变；然而，在此之后就没有保护作用了。这可能是因为已知C57Bl/6J小鼠在9个月大时会因基因因素患上OA。体育活动可能对这种遗传易感性没有影响。需要对没有OA遗传易感性的小鼠进行进一步研究。
