Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Vet Parasitol. 2014 Jun 16;203(1-2):43-50. doi: 10.1016/j.vetpar.2014.02.047. Epub 2014 Mar 1.
Fasciola gigantica is a parasitic helminth that predominantly infects the liver and bile ducts of cattle and causes great losses of cattle production in the southwestern regions of Iran. The purpose of the present study was to find out the possible relationship between the extent of liver destruction and oxidative stress by estimating the level of MDA, and SOD and GPX enzymes in the liver of cattle infected with F. gigantica. Studies were carried out on 49 infected and 20 healthy livers. Based on the results, the SOD activity of the infected livers was substantially lower than those of the healthy ones. As in the livers with more damage, a significant decrease was observed in the SOD activity. Although the GPX activity of the control livers and the livers with low damage showed no statistically significant variation this value was significantly different between the livers with high injury and the control ones. The MDA level of the infected animals was significantly higher than the non-infected animals. In addition, the level of MDA production showed a parallel correlation with the severity of infection. By histological examination, the lesions in infected livers were varied from tortuous, organized fibrotic areas in or around the hepatic lobules, cirrhosis, and chronic catarrhal cholangitis to fresh migratory tunnels filled with RBC and eosinophils. Multifocal and relatively homogenous populations of densely packed spindle cells with blunt-ended nuclei, arranged in broad interlacing fascicles were noted in samples of 10 infected livers. Based on the histological features by routine and special staining and the results of the immune labeling, the tumors were diagnosed as hepatic leiomyoma. To the best of our knowledge, this is the first report on the concomitant occurrence of Fasciola gigantica infection and leiomyoma in cattle. Therefore, there is considerable evidence indicating that the severity of hepatic damage in fasciolosis is causally associated with the extent of intrahepatic oxidative stress. Future research is necessary in order to clarify the complex host-parasite interactions and to better define the oncogenic implications of F. gigantica infection.
肝片形吸虫是一种寄生性蠕虫，主要感染牛的肝脏和胆管，导致伊朗西南部地区的牛生产损失巨大。本研究的目的是通过估计 MDA 水平以及肝脏中 SOD 和 GPX 酶的水平，找出肝片形吸虫感染牛的肝破坏程度与氧化应激之间的可能关系。研究对象为 49 例感染肝片形吸虫的牛和 20 例健康牛的肝脏。结果表明，感染牛肝脏的 SOD 活性明显低于健康牛肝脏。在肝损伤更严重的情况下，SOD 活性显著降低。虽然对照组和轻度损伤组的 GPX 活性没有统计学差异，但高损伤组与对照组之间存在显著差异。感染动物的 MDA 水平明显高于未感染动物。此外，MDA 生成水平与感染的严重程度呈平行相关。组织学检查发现，感染肝脏的病变从肝小叶内或周围曲折、有组织的纤维性区域、肝硬化和慢性卡他性胆管炎到充满 RBC 和嗜酸性粒细胞的新鲜移行性隧道不等。在 10 例感染肝脏的样本中观察到多灶性和相对均匀的密集排列的梭形细胞群体，细胞核钝端，排列在宽阔的交织束中。根据常规和特殊染色的组织学特征以及免疫标记的结果，诊断为肝平滑肌瘤。据我们所知，这是首例关于牛肝片形吸虫感染和平滑肌瘤同时发生的报道。因此，有相当多的证据表明，在 Fasciola 病中，肝损伤的严重程度与肝内氧化应激的程度有关。为了阐明复杂的宿主-寄生虫相互作用，并更好地定义肝片形吸虫感染的致癌意义，需要进一步研究。
