Młochów Research Center, Plant Breeding and Acclimatization Institute - National Research Institute, Platanowa Street 19, PL-05-831 Młochów, Poland.
Department of Plant Genetics, Breeding & Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Nowoursynowska Street 159, PL-02-776 Warsaw, Poland.
Virus Res. 2014 Jun 24;185:110-3. doi: 10.1016/j.virusres.2014.02.019. Epub 2014 Mar 15.
Four tobacco rattle virus (TRV) isolates were identified from tobacco bait seedlings planted in soil samples from Polish potato fields. Sequence analysis of the genomic RNA1 of the isolates revealed significant similarity to the isolates Ho and AL recently found in Germany. Multiple sequence alignments of the genomic RNA2 indicated that the two isolates from northern Poland (Deb57 and Slu24) are in a cluster with the isolates PSG and PLB found in the Netherlands. The remaining two isolates, from central Poland (11r21 and Mlo7), are in a distinct group with the unique isolate SYM found in England. The RNA2 sequences of the studied isolates range from 1998 nt to 2739 nt in length, and all carry deletions of the 2b and/or 2c genes. The isolate Mlo7 has an atypical RNA2 structure, having its cp gene located in its central region.
从波兰马铃薯田中采集的土壤样本中种植的烟草诱饵苗中鉴定出了四个烟草曲叶病毒（TRV）分离株。对分离株的基因组 RNA1 的序列分析表明，它们与最近在德国发现的 Ho 和 AL 分离株具有显著的相似性。对基因组 RNA2 的多个序列比对表明，来自波兰北部的两个分离株（Deb57 和 Slu24）与在荷兰发现的 PSG 和 PLB 分离株聚集在一起。其余两个分离株，来自波兰中部（11r21 和 Mlo7），与在英国发现的独特分离株 SYM 形成独特的群体。研究中分离株的 RNA2 序列长度在 1998nt 到 2739nt 之间，并且都带有 2b 和/或 2c 基因的缺失。分离株 Mlo7 的 RNA2 结构异常，其 cp 基因位于其中心区域。
