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工程化核糖体渗漏扫描和上游开放阅读框以精确控制蛋白质翻译。

Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation.

作者信息

Ferreira Joshua P, Noderer William L, Diaz de Arce Alexander J, Wang Clifford L

机构信息

Department of Chemical Engineering; Stanford University; Stanford, CA USA.

出版信息

Bioengineered. 2014 May-Jun;5(3):186-92. doi: 10.4161/bioe.27607. Epub 2014 Jan 14.

DOI:10.4161/bioe.27607
PMID:24637490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4101011/
Abstract

We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-translational control. Finally, we discuss opportunities to investigate and potentially engineer gene-specific translational responses to cellular stress.

摘要

我们利用上游开放阅读框（uORF）来系统地调节重组蛋白的翻译水平。我们展示了指导该技术开发的设计原则，并提供了可能有助于他人将合成uORF应用于自身研究的信息。我们还报告了其在我们自身研究项目中的最新应用，包括uORF与翻译起始位点（TIS）工程与小分子诱导的翻译后控制的结合。最后，我们讨论了研究并可能设计针对细胞应激的基因特异性翻译反应的机会。

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