Urch U A, Hedrick J L
Department of Biochemistry and Biophysics, University of California, Davis.
Biol Chem Hoppe Seyler. 1988 Aug;369(8):727-32. doi: 10.1515/bchm3.1988.369.2.727.
Carbohydrate-protein interactions are known to be important in gamete interactions. We therefore investigated the inhibition of boar sperm acrosin amidase activity by carbohydrates. The sulfated polysaccharides fucoidan and dextran sulfate inhibited amide hydrolysis whereas dextran and various monosaccharides did not inhibit acrosin amidase activity. The kinetics of the inhibition corresponded to those characteristic when multiple forms of an enzyme are present. Such a kinetic result was consistent with the presence of the known autolytically produced forms of acrosin. It was previously shown that sulfated polysaccharides inhibit sperm-egg binding and that acrosin binds carbohydrate. We propose that the sulfated polysaccharide inhibition of acrosin amidase activity observed here is causally related to the previously observed sulfated polysaccharide inhibition of sperm binding to the zona pellucida.
已知碳水化合物与蛋白质的相互作用在配子相互作用中很重要。因此，我们研究了碳水化合物对猪精子顶体蛋白酶酰胺酶活性的抑制作用。硫酸化多糖岩藻依聚糖和硫酸葡聚糖抑制酰胺水解，而葡聚糖和各种单糖则不抑制顶体蛋白酶酰胺酶活性。抑制动力学与存在多种形式酶时的特征相符。这样的动力学结果与已知的顶体蛋白酶自溶产生的形式一致。先前已表明硫酸化多糖抑制精卵结合，且顶体蛋白酶能结合碳水化合物。我们提出，此处观察到的硫酸化多糖对顶体蛋白酶酰胺酶活性的抑制作用与先前观察到的硫酸化多糖抑制精子与透明带结合存在因果关系。
