Wen Zhang, Abdalla Hassan E, Zhen Huang
Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA.
Sci China Chem. 2013 Mar;56(3):273-278. doi: 10.1007/s11426-012-4800-x.
The selenium derivatization of nucleic acids and nucleic acid-protein complexes has provided a powerful tool to solve phase problem in X-ray crystallography. Selenium atoms in the nucleotides can serve as fine scattering centers in crystal diffraction. Towards the synthesis of multiple selenium atom-containing nucleotides, which offers strong phasing power to facilitate crystal structure determination, we report here the synthesis of the thymidine analogue containing two Se atoms in one nucleobase. The novel Se-containing nucleoside and oligonucleotide DNAs were synthesized and found with the red-shifted UV spectrum and yellow color. Their unique properties are useful in phase determination, nucleic acid-based detection as well as spectroscopic studies of nucleic acids and nucleic acid-protein complexes.
核酸及核酸 - 蛋白质复合物的硒衍生化提供了一种强大的工具，用于解决X射线晶体学中的相位问题。核苷酸中的硒原子可作为晶体衍射中的精细散射中心。为了合成具有强大相位能力以促进晶体结构测定的含多个硒原子的核苷酸，我们在此报告了一种在一个核苷酸碱基中含有两个硒原子的胸腺嘧啶类似物的合成。合成了新型含硒核苷和寡核苷酸DNA，发现它们具有红移的紫外光谱和黄色。它们的独特性质在相位测定、基于核酸的检测以及核酸和核酸 - 蛋白质复合物的光谱研究中很有用。
