睾丸癌中Toll样受体（TLRs）的表达：一项病例对照研究。
The expression of Toll-Like Receptors (TLRs) in testicular cancer: A case control study.
作者信息
Shapouri Farnaz, Saeidi Shaghayegh, Ashrafi Kakhki Sara, Pouyan Omid, Amirchaghmaghi Elham, Aflatoonian Reza
机构信息
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
出版信息
Iran J Reprod Med. 2013 Nov;11(11):919-24.
BACKGROUND
It has been suggested that malfunction of immune system may causes testicular cancer. Recently, our understanding of innate immune system has been expanded, by discovery of "Toll-Like Receptors" (TLRs). Some studies have shown that polymorphisms of TLR2 and 4 may affect on the risk of cancer. Also, the role of TLRs 3 and 9 have been shown in apoptosis and metastasis of cancer cells in animal models.
OBJECTIVE
Little information is available about the influence of innate immunity on testicular malignancy. Therefore, expression of TLRs 2, 3, 4 and 9 as main components of innate immunity has been investigated in this study.
MATERIALS AND METHODS
In this case control study, TLRs gene expression was examined by RT-PCR in normal testis and testicular cancer tissues. Real time quantitative PCR (Q-PCR) analysis was used to compare the relative expression of TLRs between the samples.
RESULTS
mRNAs of TLR 2, 3, 4 and 9 were expressed in all normal and cancer samples. Q-PCR reveals that cancer samples had stronger expression of these genes compared with normal ones.
CONCLUSION
It seems that the different TLRs expression in testicular cancer cells may contribute to extensive signaling pathways involved in carcinogenesis.
背景
有观点认为免疫系统功能失调可能导致睾丸癌。最近，通过发现“Toll样受体”（TLRs），我们对先天免疫系统的理解得到了扩展。一些研究表明，TLR2和TLR4的多态性可能会影响患癌风险。此外，在动物模型中，TLR3和TLR9在癌细胞的凋亡和转移中也发挥了作用。
目的
关于先天免疫对睾丸恶性肿瘤的影响，目前所知甚少。因此，本研究调查了作为先天免疫主要组成部分的TLR2、TLR3、TLR4和TLR9的表达情况。
材料与方法
在这项病例对照研究中，通过逆转录聚合酶链反应（RT-PCR）检测正常睾丸组织和睾丸癌组织中TLRs基因的表达。采用实时定量聚合酶链反应（Q-PCR）分析比较样本之间TLRs的相对表达。
结果
TLR2、TLR3、TLR4和TLR9的信使核糖核酸（mRNA）在所有正常和癌组织样本中均有表达。Q-PCR显示，与正常样本相比，癌组织样本中这些基因的表达更强。
结论
睾丸癌细胞中不同的TLRs表达似乎可能促成了参与致癌过程的广泛信号通路。
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