在4-6摄氏度保存的死亡小鼠中附睾精子的活力、运动能力和DNA完整性。
The epididymal sperm viability, motility and DNA integrity in dead mice maintained at 4-6oC.
作者信息
Golshan Iranpour Farhad, Rezazadeh Valojerdi Mojtaba
机构信息
Department of Biomedical Sciences, Anatomical Sciences Section, Isfahan University of Medical Sciences, Isfahan, Iran. ; Department of Anatomy, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Embryology, Royan Institute, Tehran, Iran.
出版信息
Iran J Reprod Med. 2013 Mar;11(3):195-200.
BACKGROUND
When male animals die, spermatozoa within the body of animal will be degenerated. Because of unique chromatin structure of sperm, maybe this degeneration is different from other cells. However there is not any research which considered directly the integrity of sperm DNA by keeping the cadaver in refrigerator.
OBJECTIVE
The aim of this study was to assess viability, total motility and DNA integrity of sperm cells after death.
MATERIALS AND METHODS
In this experimental study, 24 male Swiss white mice were killed by cervical dislocation and then kept in refrigerator (4-6(o)C) for up to 12 days. On the 0 (immediately after death as control group), 1(st), 2(nd), 3(rd), 5(th), 7(th), 10(th) and the 12(th) days after death cauda epididymides were removed and squeezed in Ham's F10 medium. The proportion of viable, motile and double stranded DNA spermatozoa was examined. Viability and DNA integrity of sperm cells were examined consecutively by eosin nigrosin and acridine orange stainings.
RESULTS
The data obtained from this study showed that viability and total motility of sperm cells were significantly decreased during 12 days after death (p<0.001). In contrast with viability and motility, DNA integrity was without significant changes (even 12 days after death).
CONCLUSION
This study suggests that integrity of sperm DNA would not change even after 12 days after death if the cadaver kept in refrigerator.
背景
雄性动物死亡后，其体内的精子会发生退化。由于精子独特的染色质结构，这种退化可能与其他细胞不同。然而，尚无研究通过将尸体保存在冰箱中来直接考虑精子DNA的完整性。
目的
本研究旨在评估死亡后精子细胞的活力、总运动能力和DNA完整性。
材料与方法
在本实验研究中，24只雄性瑞士小白鼠通过颈椎脱臼处死，然后保存在冰箱（4-6℃）中长达12天。在死亡后的第0天（死后立即作为对照组）、第1天、第2天、第3天、第5天、第7天、第10天和第12天，取出附睾尾并在哈姆F10培养基中挤压。检查活精子、活动精子和双链DNA精子的比例。通过伊红黑色素和吖啶橙染色连续检查精子细胞的活力和DNA完整性。
结果
本研究获得的数据表明，死亡后12天内精子细胞的活力和总运动能力显著下降（p<0.001）。与活力和运动能力相反，DNA完整性没有显著变化（即使在死亡12天后）。
结论
本研究表明，如果尸体保存在冰箱中，即使在死亡12天后精子DNA的完整性也不会改变。
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