Hayashi T, Naruse H, Matsuda F, Iida Y
National Centre of Neurology and Psychiatry, Tokyo, Japan.
J Chromatogr. 1988 Jul 15;428(2):209-19. doi: 10.1016/s0378-4347(00)83911-0.
Sensitive methods for the determination of deuterated and non-deuterated indole-3-acetic acid and 5-hydroxyindole-3-acetic acid by combined capillary gas chromatography-negative-ion chemical ionization mass spectrometry were developed. Indole-3-acetic and 5-hydroxyindole-3-acetic acids were converted into pentafluorobenzyl and trifluoroacetylmethyl derivatives, respectively, after pre-purification by high-performance liquid chromatography. These derivatives were separated by gas chromatography and determined by selected ion monitoring. In the determinations, indole-3-acetic-2,2,2',4',5',6',7'-d7 acid and 5-hydroxyindole-3-acetic-3,3-d2 acid were used as internal standards. The methods developed in this work were used for the determination of deuterated and non-deuterated indole-3-acetic acid and 5-hydroxyindole-3-acetic acid in human urine samples collected before and after administration of L-tryptophan-3,3-d2.
开发了通过毛细管气相色谱-负离子化学电离质谱联用测定氘代和非氘代吲哚-3-乙酸及5-羟基吲哚-3-乙酸的灵敏方法。吲哚-3-乙酸和5-羟基吲哚-3-乙酸在通过高效液相色谱进行预纯化后，分别转化为五氟苄基和三氟乙酰甲基衍生物。这些衍生物通过气相色谱分离并通过选择离子监测进行测定。在测定中，吲哚-3-乙酸-2,2,2',4',5',6',7'-d7酸和5-羟基吲哚-3-乙酸-3,3-d2酸用作内标。本研究中开发的方法用于测定服用L-色氨酸-3,3-d2前后采集的人体尿液样本中的氘代和非氘代吲哚-3-乙酸及5-羟基吲哚-3-乙酸。
