Rafols J A, Cheng H W, McNeill T H
Department of Anatomy and Cell Biology, Wayne State University, Detroit, Michigan 48201.
J Comp Neurol. 1989 Jan 8;279(2):212-27. doi: 10.1002/cne.902790205.
The Van der Loos modification of the Golgi-Cox method and morphometric analyses were used to study the neuronal types in the striatum of adult (3, 6, and 10 months) and aged (20, 25, and 30 months) C57BL/6N mice. In adult mice six types of striatal neurons were distinguished primarily on the basis of the morphology of their cell body and dendrites. Each of these types was compared with morphologically similar neurons from previous Golgi classifications in other species and discussed within the framework of recent immunocytochemical work. With similar methods the age-related changes occurring on the dendrites of three of the six striatal types were also analyzed. In the medium-sized neuron with spine-laden dendrites, various dendritic tree shapes and sizes were distinguished in all age groups studied. Qualitative observations as well as measurements of total dendritic length per cell suggested that the dendrites in this type may both grow and regress throughout the life span, although signs of dendritic atrophy and regression were observed only in the aged groups. In the other two types of neuron, one a medium aspiny cell with thin varicose dendrites and the other a large spiny neuron with many dendrites, measurements of total dendritic lengths revealed sustained growth of the tree well into advanced age, followed by moderate regression in the oldest groups. The present findings also indicate that the dendrites of each type of striatal neuron follow unique temporal patterns of growth and regression during the life span of the mouse.
采用范德卢斯改良的高尔基-考克斯方法和形态计量分析，研究成年（3、6和10个月）及老年（20、25和30个月）C57BL/6N小鼠纹状体中的神经元类型。在成年小鼠中，主要根据细胞体和树突的形态区分出六种类型的纹状体神经元。将每种类型与其他物种先前高尔基分类中形态相似的神经元进行比较，并在近期免疫细胞化学研究的框架内进行讨论。采用类似方法，还分析了六种纹状体类型中的三种在树突上发生的与年龄相关的变化。在具有充满棘突树突的中型神经元中，在所有研究的年龄组中区分出了各种不同形状和大小的树突树。定性观察以及每个细胞总树突长度的测量表明，尽管仅在老年组中观察到树突萎缩和退化的迹象，但这种类型的树突在整个生命周期中可能既有生长也有退化。在另外两种神经元类型中，一种是具有细静脉曲张样树突的中型无棘细胞，另一种是具有许多树突的大型棘突神经元，总树突长度的测量显示，树突在高龄期仍持续生长，随后在最老的组中出现适度退化。目前的研究结果还表明，在小鼠的生命周期中，每种类型的纹状体神经元的树突遵循独特的生长和退化时间模式。
