Squadrito Francesco, Bitto Alessandra, Irrera Natasha, Minutoli Letteria, Caccia Paolo, Altavilla Domenica
Ital J Anat Embryol. 2013;118(1 Suppl):66-70.
Diabetic mice are characterized by an altered expression pattern of VEGF, and impaired vasculogenesis during healing. We investigated the effects of porcine derived relaxin in diabetes-related wound healing defects in genetically diabetic mice. An incisional wound model was produced on the back of female diabetic C57BL/KsJ-m+/+Lept(db) (db+/db+) mice and their normal littermates (db+/+m). Animals were treated daily with RLX (25 microg mouse/day subcutaneously) or its vehicle. Mice were killed on days 3, 6 and 12 after skin injury for measurements of vascular-endothelial-growth-factor (VEGF) mRNA and protein synthesis. Furthermore, we evaluated wound-breaking strength, histological changes, and angiogenesis at day 12. At day 6, RLX administration resulted in an increase in VEGF mRNA expression and protein wound content. Furthermore the histological evaluation indicated that RLX improved the impaired wound healing, and increased wound breaking strength at day 12 in diabetic mice. Immunohistochemistry showed that RLX in diabetic animals augmented new vessel formation. These data strongly suggest that RLX may have a potential application in diabetes-related wound disorders.
糖尿病小鼠的特征是血管内皮生长因子（VEGF）表达模式改变，愈合过程中的血管生成受损。我们研究了猪源松弛素对遗传性糖尿病小鼠糖尿病相关伤口愈合缺陷的影响。在雌性糖尿病C57BL/KsJ-m+/+Lept(db)（db+/db+）小鼠及其正常同窝小鼠（db+/+m）的背部制作了一个切口伤口模型。动物每天接受RLX（25微克/小鼠/天，皮下注射）或其载体治疗。在皮肤损伤后第3、6和12天处死小鼠，以测量血管内皮生长因子（VEGF）mRNA和蛋白质合成。此外，我们在第12天评估了伤口抗张强度、组织学变化和血管生成。在第6天，给予RLX导致VEGF mRNA表达和伤口蛋白质含量增加。此外，组织学评估表明，RLX改善了受损的伤口愈合，并在第12天增加了糖尿病小鼠的伤口抗张强度。免疫组织化学显示，糖尿病动物体内的RLX增强了新血管形成。这些数据有力地表明，RLX可能在糖尿病相关伤口疾病中具有潜在应用。
