Dong Yanpeng, Fan Guoqiang, Zhao Zhenli, Deng Minjie
Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, China.
College of Forestry, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, China.
Int J Mol Sci. 2014 Mar 17;15(3):4583-607. doi: 10.3390/ijms15034583.
The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might be involved in this plant's response to water deficit. Potted seedlings were subjected to well-watered conditions and drought stress, respectively. More than 290 million raw transcript reads were assembled into 111,660 unigenes, with a mean length of 1013 bp. Clusters of orthologous groups, gene ontology and the Kyoto Encyclopedia of Genes and Genomes annotations analyses were performed on the unigenes. Many differentially expressed genes and several metabolic pathways were identified. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of 14 genes. Our study identified altered gene expression in P. australis induced by drought stress and provided a comprehensive map of drought-responsive genes and pathways in this species. To our knowledge, this is the first publicly available global transcriptome study of P. australis. This study provides a valuable genetic resource for this species.
人们对南方泡桐对干旱的响应和适应性仍知之甚少。为了研究这个问题，利用Illumina Genome Analyzer IIx分析技术，对处于水分胁迫条件下的四个南方泡桐种源（两个二倍体和另外两个同源四倍体）进行了转录组分析。本研究旨在鉴定南方泡桐代谢途径中可能参与该植物对水分亏缺响应的基因。将盆栽幼苗分别置于充分浇水条件和干旱胁迫条件下。超过2.9亿条原始转录本读数被组装成111,660个单基因，平均长度为1013 bp。对这些单基因进行了直系同源基因簇、基因本体和京都基因与基因组百科全书注释分析。鉴定出许多差异表达基因和几个代谢途径。采用定量实时聚合酶链反应验证了14个基因的表达模式。我们的研究确定了干旱胁迫诱导的南方泡桐基因表达变化，并提供了该物种干旱响应基因和途径的全面图谱。据我们所知，这是首次公开的南方泡桐全球转录组研究。该研究为该物种提供了宝贵的遗传资源。
