De Bock Lies, Boussery Koen, De Bruyne Ruth, Van Winckel Myriam, Stephenne Xavier, Sokal Etienne, Van Bocxlaer Jan
Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
Biopharm Drug Dispos. 2014 Jul;35(5):308-12. doi: 10.1002/bdd.1895. Epub 2014 Apr 1.
The microsomal protein per gram of liver (MPPGL) is an important scaling factor in the in vitro-in vivo extrapolation of metabolic data obtained in liver microsomes. This study aimed to determine the MPPGL in four biliary atresia patients (0.6-1.6 years old) undergoing liver transplantation, as it is known that the MPPGL is affected by age and possibly by liver disease. Due to the presence of bilirubin in the homogenates and microsomes, the NADPH-cytochrome reductase activity was used to determine the recovery factor, rather than methods using the dithionite difference spectrum. A mean value of 18.73 (± 2.82) mg/g (geometric mean ± SD, n = 4) was observed, which is lower than the expected MPPGL based on the age of the patients (26.60 ± 0.40 mg/g). This suggests a decreased amount of microsomal protein in the livers of biliary atresia patients. Moreover, no differences in MPPGL between different zones of the liver could be detected.
每克肝脏的微粒体蛋白（MPPGL）是在体外-体内外推肝脏微粒体中获得的代谢数据时的一个重要缩放因子。本研究旨在确定四名接受肝移植的胆道闭锁患者（0.6 - 1.6岁）的MPPGL，因为已知MPPGL受年龄影响，也可能受肝脏疾病影响。由于匀浆和微粒体中存在胆红素，使用NADPH - 细胞色素还原酶活性来确定回收因子，而不是使用连二亚硫酸盐差示光谱的方法。观察到平均值为18.73（±2.82）mg/g（几何平均值±标准差，n = 4），这低于基于患者年龄预期的MPPGL（26.60±0.40 mg/g）。这表明胆道闭锁患者肝脏中的微粒体蛋白量减少。此外，未检测到肝脏不同区域之间MPPGL的差异。
