Rastinejad F, Polverini P J, Bouck N P
Department of Microbiology-Immunology and Cancer Center, Northwestern University Medical Schools, Chicago, Illinois 60611.
Cell. 1989 Feb 10;56(3):345-55. doi: 10.1016/0092-8674(89)90238-9.
An inhibitor has been identified in the conditioned medium of hamster cells and hamster-human hybrids that suppresses neovascularization in vivo in the rat cornea. Inhibitory activity was tightly linked to the presence of an active cancer suppressor gene in transformants and revertants, in segregating hybrids, and in temperature-limited transformants. It copurified with a approximately 140 kd glycoprotein. Polyclonal antiserum raised against the purified preparation recognized a 140 kd protein in conditioned medium and was able to adsorb out all antiangiogenic activity. These results define the control of the activity of an inhibitor of neovascularization as one function of the cancer suppressor gene active in BHK21/cl13 cells and simultaneously identify a new inhibitor of angiogenesis, a process vital to the growth of solid tumors.
在仓鼠细胞和仓鼠 - 人杂交细胞的条件培养基中已鉴定出一种抑制剂，它可抑制大鼠角膜体内的新血管形成。在转化体、回复体、分离的杂交细胞以及温度限制转化体中，抑制活性与活性抑癌基因的存在紧密相关。它与一种约140 kd的糖蛋白共纯化。针对纯化制剂产生的多克隆抗血清可识别条件培养基中的一种140 kd蛋白质，并能够去除所有抗血管生成活性。这些结果将新血管形成抑制剂活性的控制定义为在BHK21/cl13细胞中起作用的抑癌基因的一项功能，同时鉴定出一种新的血管生成抑制剂，而血管生成过程对实体瘤的生长至关重要。
