Lerner K, Goldstein P
Department of Biological Sciences, University of Texas, El Paso 79968.
Cytobios. 1988;55(220):51-61.
Decondensed chromatin regions have been described along the pachytene chromosomes of Caenorhabditis elegans (Goldstein, 1985). Regions of similar appearance, but not in pachytene chromosomes, have been shown to be transcriptionally active in other organisms (Angelier et al., 1979; Nagl, 1985). Incorporation of tritiated-uridine and electron microscopy autoradiography were utilized, in the present study, to determine if RNA transcription occurred along meiotic chromosomes of C. elegans. This study presents new evidence that RNA transcription occurs in highly defined regions along pachytene bivalents.
在秀丽隐杆线虫的粗线期染色体上已发现解聚的染色质区域（戈尔茨坦，1985年）。在其他生物体中，已发现外观相似但不在粗线期染色体上的区域具有转录活性（安热利耶等人，1979年；纳格尔，1985年）。在本研究中，利用氚标记尿苷掺入法和电子显微镜放射自显影术来确定秀丽隐杆线虫减数分裂染色体上是否发生RNA转录。这项研究提供了新的证据，表明RNA转录发生在粗线期二价体的高度特定区域。
