U4和U6小核核糖核蛋白颗粒。2. 它们参与前体信使核糖核酸剪接的证据。
U4 and U6 small nuclear ribonucleoprotein particles. 2. Evidence for their involvement in pre-mRNA splicing.
作者信息
Pironcheva G, Todorov I, Lesseva M, Russev G
机构信息
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
出版信息
Cytobios. 1988;55(220):7-16.
PMID:2464460
Abstract
The in vitro splicing of pre-mRNA of the human beta-globin gene in the presence of HeLa cell nuclear extract was investigated. Splicing was inhibited by auto-antibodies against U4 and U6 snRNP particles. No intermediates or products of the splicing reaction were evident in the presence of antibodies against U4 and U6 snRNPs which suggests their involvement in pre-mRNA splicing.
摘要
研究了在HeLa细胞核提取物存在下人类β-珠蛋白基因前体mRNA的体外剪接。针对U4和U6 snRNP颗粒的自身抗体抑制了剪接。在存在针对U4和U6 snRNPs的抗体时，剪接反应的中间体或产物不明显，这表明它们参与了前体mRNA的剪接。
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