Makin C A, Bobrow L G, Nicholls R J
Imperial Cancer Research Fund, Lincolns Inn Fields, London, United Kingdom.
Dis Colon Rectum. 1989 Feb;32(2):99-102. doi: 10.1007/BF02553819.
Employing the monoclonal antibody CAM5.2, the sensitivity of immunohistologic staining was compared with conventional hematoxylin and eosin staining for detection of lymph-node metastases from large-bowel cancer. Ten patients who died unexpectedly early from recurrent disease were selected; 86 lymph nodes were examined. Where metastases were located they were readily identified by both methods but when the original lymph-node sections were reviewed, attention was drawn to two metastatic deposits that had been overlooked previously. These results suggest that immunohistologic techniques offer no advantage in the identification of lymph-node metastases in large-bowel cancer.
采用单克隆抗体CAM5.2，将免疫组织化学染色检测大肠癌淋巴结转移的敏感性与传统苏木精和伊红染色进行比较。选择了10例因复发疾病意外早亡的患者；检查了86个淋巴结。在有转移的部位，两种方法都能很容易地识别出转移灶，但在复查原始淋巴结切片时，发现了两个之前被忽视的转移灶。这些结果表明，免疫组织学技术在识别大肠癌淋巴结转移方面没有优势。
