Medkova A Iu, Siniashina L N, Rumiantseva Iu P, Voronina O L, Kunda M S, Karataev G I
Mol Gen Mikrobiol Virusol. 2013(4):22-6.
The duration of the persistence and dynamics of accumulation of insertion bvg- Bordetella pertussis mutants were studied in lungs of laboratory mice after intranasal and intravenous challenge by virulent bacteria of the causative agent of whooping cough. The capability of the virulent B. pertussis bacteria to long-term persistence in the body of mice was tested. Using the real-time PCR approximately hundred genome equivalents of the B. pertussis DNA were detected in lungs of mice in two months after infection regardless of the way of challenge. Using the bacterial test bacteria were identified during only four weeks after challenge. Bvg- B. pertussis avirulent mutants were accumulated for the infection time. The percentage of the avirulent bacteria in the B. pertussis population reached 50% in 7-9 weeks after challenge. The obtained results show that the laboratory mice can be used for study of the B. pertussis insertion mutant formation dynamics in vivo and confirm the hypothesis about insertional bvg- B. pertussis virulent mutants accumulation during development of pertussis infection in human.
在通过鼻内和静脉注射百日咳病原体的强毒细菌对实验小鼠进行攻击后，研究了插入型bvg - 百日咳博德特氏菌突变体在小鼠肺部持续存在的时间和积累动态。测试了强毒百日咳博德特氏菌在小鼠体内长期持续存在的能力。使用实时PCR，无论攻击方式如何，在感染后两个月的小鼠肺部均检测到约一百个基因组当量的百日咳博德特氏菌DNA。使用细菌检测法，仅在攻击后四周内鉴定出细菌。bvg - 百日咳博德特氏菌无毒突变体在感染期间积累。在攻击后7 - 9周，百日咳博德特氏菌群体中无毒细菌的百分比达到50%。获得的结果表明，实验小鼠可用于研究百日咳博德特氏菌插入突变体在体内的形成动态，并证实了关于在人类百日咳感染发展过程中插入型bvg - 百日咳博德特氏菌强毒突变体积累的假说。
