Boyce S T, Christianson D J, Hansbrough J F
Department of Surgery, University of California San Diego Medical Center 92103.
J Biomed Mater Res. 1988 Oct;22(10):939-57. doi: 10.1002/jbm.820221008.
Collagen and glycosaminoglycan (GAG) dermal skin substitutes (membranes) were studied as substrates for cultured human epidermal keratinocytes. Structure of dermal substitutes was optimized for pore size to promote ingrowth of fibrovascular tissue from the wound bed and for culture of human keratinocytes of the membrane's surface. Pore size of the freeze-dried material was regulated by control of the temperature of freezing between -50 degrees C and -20 degrees C and by concentration of starting materials between 0.17% and 1.62% wt/vol. A nonporous surface of collagen-GAG was laminated to the membranes to provide a planar substrate for cultured epidermal keratinocytes. Thickness of dermal substitutes was regulated by control of the volume and concentration of starting materials. Biotin was conjugated to solubilized collagen for binding with avidin of specific quantities of biologically active molecules. The optimized membranes are suitable substrates for the culture of human epidermal keratinocytes, and together with the cells yield a composite material that is histologically similar to skin.
研究了胶原蛋白和糖胺聚糖（GAG）真皮皮肤替代物（膜）作为培养人表皮角质形成细胞的底物。对真皮替代物的结构进行了优化，以使其孔径能够促进伤口床的纤维血管组织向内生长，并有利于在膜表面培养人角质形成细胞。通过控制-50℃至-20℃之间的冷冻温度以及起始材料0.17%至1.62%重量/体积的浓度来调节冻干材料的孔径。将无孔的胶原蛋白-GAG表面层压到膜上，为培养的表皮角质形成细胞提供一个平面底物。通过控制起始材料的体积和浓度来调节真皮替代物的厚度。将生物素与可溶解的胶原蛋白结合，以便与特定数量生物活性分子的抗生物素蛋白结合。优化后的膜是培养人表皮角质形成细胞的合适底物，并且与这些细胞一起产生一种组织学上与皮肤相似的复合材料。
