使用改良空斑形成试验对感染性人类免疫缺陷病毒进行定量分析。
Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay.
作者信息
Kiernan R E, McPhee D A, Doherty R R
机构信息
NHMRC Special Unit for AIDS Virology, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.
出版信息
J Virol Methods. 1988 Dec;22(2-3):303-8. doi: 10.1016/0166-0934(88)90112-7.
This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids.
本报告描述了一种改良的蚀斑形成试验，该试验已用于定量细胞培养上清液中具有传染性的人类免疫缺陷病毒（HIV）。通过蚀斑试验确定的感染单位数量与细胞培养中传统试验确定的50%感染剂量密切相关。相比之下，与颗粒相关的逆转录酶（RT）活性与培养上清液中存在的传染性HIV数量相关性较差。
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