Bourinbaiar A S
Department of Biochemistry, NYU Medical Center, NY 10016.
Acta Virol. 1994 Feb;38(1):59-61.
The ratio of infectious to defective particles has been investigated for human immunodeficiency virus type 1 (HIV-1). Although the concentration of HIV core p24 protein defined by ELISA permits the estimation of a total number of average-sized HIV particles, it does not provide information on the numerical value of infectious particles. This problem was addressed by limiting dilution of viral supernatant derived from various infected cell lines and then comparing the total number of HIV particles to the end-point titers of viral inoculum determined by reverse transcriptase assay and virus-induced cytotoxicity. The results indicate that the ratio of infectious to defective virus particles varies for different virus strains and host cells within a range from 1:1 to 1:100, but for given virus-cell system it remains constant.
已对1型人类免疫缺陷病毒（HIV-1）的感染性颗粒与缺陷颗粒的比例进行了研究。尽管通过酶联免疫吸附测定（ELISA）确定的HIV核心p24蛋白浓度能够估算平均大小的HIV颗粒总数，但它无法提供感染性颗粒数值的相关信息。通过对源自各种感染细胞系的病毒上清液进行有限稀释，然后将HIV颗粒总数与通过逆转录酶测定和病毒诱导的细胞毒性确定的病毒接种物终点滴度进行比较，解决了这个问题。结果表明，感染性病毒颗粒与缺陷病毒颗粒的比例因不同病毒株和宿主细胞而异，范围在1:1至1:100之间，但对于特定的病毒-细胞系统，该比例保持恒定。
