针对P2碱性蛋白的单克隆抗体及其在格林-巴利综合征某些方面研究中的应用。

Monoclonal antibodies to P2 basic protein and their use in the investigation of some aspects of the Guillain-Barré syndrome.

作者信息

Luijten J A, Reijneveld-de Jong S D, Teerlink T, Camps V T, Osterhaus A D, UytdeHaag A G

机构信息

Department of Neurology, University Hospital, Utrecht, The Netherlands.

出版信息

J Neurol Sci. 1988 Sep;86(2-3):265-76. doi: 10.1016/0022-510x(88)90104-9.

DOI:10.1016/0022-510x(88)90104-9

PMID:2464668

Abstract

The production and characterization of two mouse monoclonal antibodies (MAbs) raised against human basic protein P2 is described. The two MAbs recognise two non-overlapping epitopes on P2. Using these two MAbs as a probe, a search was made for the presence of anti-idiotype (Ab2) antibodies directed against anti-P2 (Ab1) autoantibodies in sera of patients with Guillain-Barré syndrome (GBS). In sera of twenty GBS patients no such Ab2 antibodies could be demonstrated. Epitopes present on cytomegalo virus- or Epstein-Barr virus-infected cells, which may play a part in triggering GBS, were not recognised by the two MAbs.

摘要

本文描述了两种针对人碱性蛋白P2产生的小鼠单克隆抗体（MAb）的制备及特性。这两种单克隆抗体识别P2上两个不重叠的表位。以这两种单克隆抗体作为探针，在格林-巴利综合征（GBS）患者血清中寻找针对抗P2（Ab1）自身抗体的抗独特型（Ab2）抗体。在20例GBS患者的血清中未证实存在此类Ab2抗体。巨细胞病毒或EB病毒感染细胞上存在的、可能在引发GBS中起作用的表位未被这两种单克隆抗体识别。