关于夏因-达尔加诺序列在确定大肠杆菌K12中car操纵子弱起始密码子处翻译起始效率方面的作用

On the role of the Shine-Dalgarno sequence in determining the efficiency of translation initiation at a weak start codon in the car operon of Escherichia coli K12.

作者信息

Weyens G, Charlier D, Roovers M, Piérard A, Glansdorff N

机构信息

Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Belgium.

出版信息

J Mol Biol. 1988 Dec 20;204(4):1045-8. doi: 10.1016/0022-2836(88)90061-7.

DOI:10.1016/0022-2836(88)90061-7

PMID:2464695

Abstract

Translation of the carA gene is efficiently initiated at the intrinsically weak UUG codon. A single nucleotide substitution changing the Shine-Dalgarno box of carA (GGAGG) into the sequence TGAGG reduces translation of carA sevenfold. This result supports the view that extensive complementarity between the Shine-Dalgarno sequence and 16S RNA contributes significantly to the efficiency of translation when the latter starts at a weak initiation triplet.

摘要

carA基因的翻译在本质上较弱的UUG密码子处高效起始。将carA的Shine-Dalgarno框（GGAGG）改变为TGAGG的单个核苷酸替换使carA的翻译减少了七倍。这一结果支持了这样一种观点，即当翻译从一个弱起始三联体开始时，Shine-Dalgarno序列与16S RNA之间的广泛互补性对翻译效率有显著贡献。