在大肠杆菌中，有效的Shine-Dalgarno序列对lacZ mRNA稳定性是必要的，但翻译并非必需。
An efficient Shine-Dalgarno sequence but not translation is necessary for lacZ mRNA stability in Escherichia coli.
作者信息
Wagner L A, Gesteland R F, Dayhuff T J, Weiss R B
机构信息
Department of Human Genetics, University of Utah, Salt Lake City 84132.
出版信息
J Bacteriol. 1994 Mar;176(6):1683-8. doi: 10.1128/jb.176.6.1683-1688.1994.
The 5' ends of many bacterial transcripts are important in determining mRNA stability. A series of Shine-Dalgarno (SD) sequence changes showed that the complementarity of the SD sequence to the anti-SD sequence of 16S rRNA correlates with lacZ mRNA stability in Escherichia coli. Several initiation codon changes showed that an efficient initiation codon is not necessary to maintain lacZ mRNA stability. A stop codon in the 10th codon of lacZ increased mRNA stability. Therefore, ribosomal binding via the SD sequence but not translation of the coding region is necessary to maintain lacZ mRNA stability.
许多细菌转录本的5'端在决定mRNA稳定性方面很重要。一系列Shine-Dalgarno（SD）序列变化表明，SD序列与16S rRNA的反SD序列的互补性与大肠杆菌中lacZ mRNA的稳定性相关。几个起始密码子的变化表明，有效的起始密码子对于维持lacZ mRNA的稳定性并非必需。lacZ第10个密码子中的终止密码子增加了mRNA的稳定性。因此，通过SD序列进行核糖体结合而非编码区的翻译对于维持lacZ mRNA的稳定性是必要的。
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