• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

莫洛尼鼠白血病病毒逆转录酶3'区域的单突变对核糖核酸酶H活性和病毒复制的抑制作用

Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.

作者信息

Repaske R, Hartley J W, Kavlick M F, O'Neill R R, Austin J B

机构信息

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

出版信息

J Virol. 1989 Mar;63(3):1460-4. doi: 10.1128/JVI.63.3.1460-1464.1989.

DOI:10.1128/JVI.63.3.1460-1464.1989
PMID:2464706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC247852/
Abstract

Selected conserved amino acids in the putative RNase H domain of reverse transcriptase (RT) were modified in a molecularly cloned infectious provirus and in a Moloney murine leukemia virus RT expression vector by site-directed mutagenesis. Substitution of either of two conserved aspartic acid residues in proviral DNA prevented production of infectious particles in transfected NIH 3T3 cells, and the same modifications depressed RT-associated RNase H activity by more than 25-fold with little or no effect on polymerase activity.

摘要

通过定点诱变，在分子克隆的感染性前病毒和莫洛尼鼠白血病病毒逆转录酶（RT）表达载体中，对逆转录酶（RT）假定的核糖核酸酶H结构域中的选定保守氨基酸进行了修饰。前病毒DNA中两个保守天冬氨酸残基中的任何一个被取代，都会阻止转染的NIH 3T3细胞中产生感染性颗粒，并且相同的修饰使RT相关的核糖核酸酶H活性降低了25倍以上，而对聚合酶活性几乎没有影响。

相似文献

1
Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.莫洛尼鼠白血病病毒逆转录酶3'区域的单突变对核糖核酸酶H活性和病毒复制的抑制作用
J Virol. 1989 Mar;63(3):1460-4. doi: 10.1128/JVI.63.3.1460-1464.1989.
2
Mutations of the RNase H C helix of the Moloney murine leukemia virus reverse transcriptase reveal defects in polypurine tract recognition.莫洛尼鼠白血病病毒逆转录酶的核糖核酸酶H C螺旋突变揭示了多聚嘌呤序列识别缺陷。
J Virol. 2002 Aug;76(16):8360-73. doi: 10.1128/jvi.76.16.8360-8373.2002.
3
Abortive reverse transcription by mutants of Moloney murine leukemia virus deficient in the reverse transcriptase-associated RNase H function.莫洛尼鼠白血病病毒中逆转录酶相关核糖核酸酶H功能缺陷的突变体导致的流产逆转录。
J Virol. 1991 Aug;65(8):4387-97. doi: 10.1128/JVI.65.8.4387-4397.1991.
4
Amino acid substitutions away from the RNase H catalytic site increase the thermal stability of Moloney murine leukemia virus reverse transcriptase through RNase H inactivation.远离核糖核酸酶H催化位点的氨基酸替换通过使核糖核酸酶H失活来提高莫洛尼鼠白血病病毒逆转录酶的热稳定性。
Biochem Biophys Res Commun. 2014 Nov 14;454(2):269-74. doi: 10.1016/j.bbrc.2014.10.044. Epub 2014 Oct 17.
5
Site-directed mutagenesis of Moloney murine leukemia virus reverse transcriptase. Demonstration of lysine 103 in the nucleotide binding site.莫洛尼鼠白血病病毒逆转录酶的定点诱变。核苷酸结合位点中赖氨酸103的验证。
J Biol Chem. 1990 Oct 5;265(28):17162-6.
6
Preparation and characterization of the RNase H domain of Moloney murine leukemia virus reverse transcriptase.莫洛尼鼠白血病病毒逆转录酶核糖核酸酶H结构域的制备与表征
Protein Expr Purif. 2015 Sep;113:44-50. doi: 10.1016/j.pep.2015.04.012. Epub 2015 May 7.
7
Substitution of Asp114 or Arg116 in the fingers domain of moloney murine leukemia virus reverse transcriptase affects interactions with the template-primer resulting in decreased processivity.莫洛尼鼠白血病病毒逆转录酶指状结构域中Asp114或Arg116的取代会影响与模板引物的相互作用，导致持续合成能力下降。
J Mol Biol. 2001 Jan 12;305(2):341-59. doi: 10.1006/jmbi.2000.4281.
8
Defects in Moloney murine leukemia virus replication caused by a reverse transcriptase mutation modeled on the structure of Escherichia coli RNase H.基于大肠杆菌核糖核酸酶H结构构建的逆转录酶突变导致莫洛尼鼠白血病病毒复制缺陷。
J Virol. 1992 Feb;66(2):615-22. doi: 10.1128/JVI.66.2.615-622.1992.
9
Nuclease activities of Moloney murine leukemia virus reverse transcriptase. Mutants with altered substrate specificities.莫洛尼鼠白血病病毒逆转录酶的核酸酶活性。具有改变底物特异性的突变体。
J Biol Chem. 1993 Nov 5;268(31):23585-92.
10
Similarities and differences in the RNase H activities of human immunodeficiency virus type 1 reverse transcriptase and Moloney murine leukemia virus reverse transcriptase.1型人类免疫缺陷病毒逆转录酶与莫洛尼鼠白血病病毒逆转录酶核糖核酸酶H活性的异同
J Mol Biol. 1999 Dec 17;294(5):1097-113. doi: 10.1006/jmbi.1999.3325.

引用本文的文献

1
Mechanism-Based Inactivation of Ribonuclease A.基于机制的核糖核酸酶A失活
J Org Chem. 1995 Oct;60(21):6930-6936. doi: 10.1021/jo00126a051.
2
Structural and Functional Aspects of Foamy Virus Protease-Reverse Transcriptase.泡沫病毒蛋白酶-逆转录酶的结构和功能方面。
Viruses. 2019 Jul 2;11(7):598. doi: 10.3390/v11070598.
3
Role of RNase H1 in DNA repair: removal of single ribonucleotide misincorporated into DNA in collaboration with RNase H2.核糖核酸酶H1在DNA修复中的作用：与核糖核酸酶H2协同作用去除错掺入DNA中的单个核糖核苷酸。
Sci Rep. 2015 May 7;5:9969. doi: 10.1038/srep09969.
4
SiRNA-induced mutation in HIV-1 polypurine tract region and its influence on viral fitness.小干扰RNA诱导的HIV-1多聚嘌呤序列区域突变及其对病毒适应性的影响。
PLoS One. 2015 Apr 10;10(4):e0122953. doi: 10.1371/journal.pone.0122953. eCollection 2015.
5
The nature of the N-terminal amino acid residue of HIV-1 RNase H is critical for the stability of reverse transcriptase in viral particles.HIV-1核糖核酸酶H的N端氨基酸残基的性质对于逆转录酶在病毒颗粒中的稳定性至关重要。
J Virol. 2015 Jan 15;89(2):1286-97. doi: 10.1128/JVI.02312-14. Epub 2014 Nov 12.
6
Insights into the structure and activity of prototype foamy virus RNase H.原型泡沫病毒 RNase H 的结构与活性研究进展
Retrovirology. 2012 Feb 10;9:14. doi: 10.1186/1742-4690-9-14.
7
Multiple nucleotide preferences determine cleavage-site recognition by the HIV-1 and M-MuLV RNases H.多种核苷酸偏好决定了 HIV-1 和 M-MuLV RNases H 的切割位点识别。
J Mol Biol. 2010 Mar 19;397(1):161-78. doi: 10.1016/j.jmb.2010.01.059. Epub 2010 Feb 1.
8
Preferred sequences within a defined cleavage window specify DNA 3' end-directed cleavages by retroviral RNases H.在定义的切割窗口内的优选序列指定了逆转录病毒核糖核酸酶H对DNA 3'端的定向切割。
J Biol Chem. 2009 Nov 20;284(47):32225-38. doi: 10.1074/jbc.M109.043158. Epub 2009 Sep 24.
9
Effects of mutations in the G tract of the human immunodeficiency virus type 1 polypurine tract on virus replication and RNase H cleavage.人类免疫缺陷病毒1型多聚嘌呤序列G区突变对病毒复制及核糖核酸酶H切割的影响
J Virol. 2004 Dec;78(23):13315-24. doi: 10.1128/JVI.78.23.13315-13324.2004.
10
Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer.人类免疫缺陷病毒1型多聚嘌呤序列5'端的突变会影响核糖核酸酶H的切割特异性和病毒滴度。
J Virol. 2003 Oct;77(20):11150-7. doi: 10.1128/jvi.77.20.11150-11157.2003.

本文引用的文献

1
Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.人类成人T细胞白血病病毒：整合于白血病细胞DNA中的前病毒基因组的完整核苷酸序列。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618-22. doi: 10.1073/pnas.80.12.3618.
2
Nucleotide sequence of Rous sarcoma virus.劳氏肉瘤病毒的核苷酸序列。
Cell. 1983 Mar;32(3):853-69. doi: 10.1016/0092-8674(83)90071-5.
3
DNA sequence of the gene coding for Escherichia coli ribonuclease H.编码大肠杆菌核糖核酸酶H的基因的DNA序列。
J Biol Chem. 1983 Jan 25;258(2):1276-81.
4
Involvement of retrovirus reverse transcriptase-associated RNase H in the initiation of strong-stop (+) DNA synthesis and the generation of the long terminal repeat.逆转录病毒逆转录酶相关核糖核酸酶H参与强终止（+）DNA合成的起始及长末端重复序列的产生。
J Virol. 1984 Sep;51(3):813-21. doi: 10.1128/JVI.51.3.813-821.1984.
5
Murine leukemia virus mutant with a frameshift in the reverse transcriptase coding region: implications for pol gene structure.逆转录酶编码区发生移码突变的鼠白血病病毒：对pol基因结构的影响
J Virol. 1984 Aug;51(2):470-8. doi: 10.1128/JVI.51.2.470-478.1984.
6
Construction and analysis of deletion mutations in the pol gene of Moloney murine leukemia virus: a new viral function required for productive infection.莫洛尼鼠白血病病毒pol基因缺失突变的构建与分析：有效感染所需的一种新病毒功能
Cell. 1984 Jul;37(3):1043-52. doi: 10.1016/0092-8674(84)90439-2.
7
Sequence similarity among retroviruses--erratum.逆转录病毒之间的序列相似性——勘误
Nature. 1984;309(5970):728. doi: 10.1038/309728b0.
8
Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus.逆转录病毒逆转录酶与乙型肝炎病毒及花椰菜花叶病毒假定聚合酶之间的序列同源性。
Nature. 1983;305(5937):827-9. doi: 10.1038/305827a0.
9
Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects.逆转录参与花椰菜花叶病毒复制的研究：一个详细模型及部分方面的验证
Cell. 1983 Jul;33(3):781-9. doi: 10.1016/0092-8674(83)90020-x.
10
Mechanism of release of the avian rotavirus tRNATrp primer molecule from viral DNA by ribonuclease H during reverse transcription.禽轮状病毒tRNATrp引物分子在逆转录过程中通过核糖核酸酶H从病毒DNA上释放的机制。
Cell. 1982 Oct;30(3):797-805. doi: 10.1016/0092-8674(82)90284-7.