莫洛尼鼠白血病病毒逆转录酶3'区域的单突变对核糖核酸酶H活性和病毒复制的抑制作用
Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.
作者信息
Repaske R, Hartley J W, Kavlick M F, O'Neill R R, Austin J B
机构信息
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
出版信息
J Virol. 1989 Mar;63(3):1460-4. doi: 10.1128/JVI.63.3.1460-1464.1989.
Abstract
Selected conserved amino acids in the putative RNase H domain of reverse transcriptase (RT) were modified in a molecularly cloned infectious provirus and in a Moloney murine leukemia virus RT expression vector by site-directed mutagenesis. Substitution of either of two conserved aspartic acid residues in proviral DNA prevented production of infectious particles in transfected NIH 3T3 cells, and the same modifications depressed RT-associated RNase H activity by more than 25-fold with little or no effect on polymerase activity.
摘要
通过定点诱变，在分子克隆的感染性前病毒和莫洛尼鼠白血病病毒逆转录酶（RT）表达载体中，对逆转录酶（RT）假定的核糖核酸酶H结构域中的选定保守氨基酸进行了修饰。前病毒DNA中两个保守天冬氨酸残基中的任何一个被取代，都会阻止转染的NIH 3T3细胞中产生感染性颗粒，并且相同的修饰使RT相关的核糖核酸酶H活性降低了25倍以上，而对聚合酶活性几乎没有影响。
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