Williams Sharon, Morton Phil, Baines Dev
ProMetic BioSciences Ltd, Cambridge, UK.
Methods Mol Biol. 2014;1129:181-95. doi: 10.1007/978-1-62703-977-2_16.
Synthetic ligand affinity adsorbents offer an efficient means for purification of biopharmaceuticals. Single isomer textile dye C.I. Reactive Blue and newer ligands developed by rational design and screening of chemical combinatorial libraries based on a triazine scaffold are routinely used for the capture and purification of these proteins from engineered recombinant expression systems. Here we describe methods for the purification of recombinant human serum albumin and related fusion proteins using synthetic ligand affinity adsorbents.
合成配体亲和吸附剂为生物药物的纯化提供了一种有效方法。单一异构体纺织染料C.I.活性蓝以及基于三嗪支架通过合理设计和化学组合文库筛选开发的新型配体，常用于从工程重组表达系统中捕获和纯化这些蛋白质。本文我们描述了使用合成配体亲和吸附剂纯化重组人血清白蛋白及相关融合蛋白的方法。
