Ortmann M, Vierbuchen M, Fischer R
Institute of Pathology, University of Cologne, Köln, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(3):175-84.
The present study is aimed to gain more insight into the histochemical properties of renal oncocytomas. Ten oncocytomas and normal kidneys were investigated using several lectins (peanut agglutinin--PNA, Dolichos biflorus agglutinin--DBA and Ulex europaeus agglutinin--UEA) and antibodies against epithelial membrane antigen (EMA), Tamm-Horsfall glycoprotein (THG) and lysozyme. Lectin histochemistry revealed a characteristic binding pattern in renal oncocytomas, with strong DBA-binding and, in some cases, a weaker staining with UEA apparent in the cytoplasm of the oncocytes. PNA binding sites were evident only after enzymatic cleavage of sialic acid by neuraminidase. Comparative evaluation of normal kidneys exhibiting a strict compartmentalization of saccharide moieties in the various nephron segments revealed a similar binding pattern exclusively in interspersed collecting duct epithelium. This striking resemblance suggests that renal oncocytomas may originate from the collecting duct system. Further support for this assumption has been provided by the demonstration of strong cytoplasmic EMA reactivity in the oncocytes. In normal kidneys prominent labeling for EMA was apparent in the very same interspersed cells of the collecting ducts. THG and lysozyme failed to react in renal oncocytomas. In accordance with observations recently reported in the literature, these results clearly favor a histogenetic origin of renal oncocytomas from the collecting duct epithelium.
本研究旨在更深入地了解肾嗜酸细胞瘤的组织化学特性。使用几种凝集素（花生凝集素——PNA、双花扁豆凝集素——DBA和欧洲荆豆凝集素——UEA）以及抗上皮膜抗原（EMA）、Tamm-Horsfall糖蛋白（THG）和溶菌酶的抗体对10个嗜酸细胞瘤和正常肾脏进行了研究。凝集素组织化学显示肾嗜酸细胞瘤具有特征性的结合模式，DBA结合强烈，在某些情况下，在嗜酸细胞的细胞质中可见较弱的UEA染色。只有在神经氨酸酶对唾液酸进行酶切后，PNA结合位点才明显。对正常肾脏中不同肾单位节段糖类部分呈现严格分区的比较评估显示，仅在散在的集合管上皮中存在类似的结合模式。这种显著的相似性表明肾嗜酸细胞瘤可能起源于集合管系统。嗜酸细胞中强烈的细胞质EMA反应性进一步支持了这一假设。在正常肾脏中，在集合管相同的散在细胞中可见明显的EMA强标记。THG和溶菌酶在肾嗜酸细胞瘤中无反应。与最近文献报道的观察结果一致，这些结果明显支持肾嗜酸细胞瘤起源于集合管上皮的组织发生学观点。
