Qi Zongli, Li Yuan, Hu Jun, Guo Hua, Zhao Xiangrong, Wang Guanghua, Gao Jinwei, Hu Qiaoxia
Laboratory Center, Shaanxi Provincial People's Hospital and The Third Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710068, P.R. China.
Biomed Rep. 2013 Jan;1(1):111-114. doi: 10.3892/br.2012.22. Epub 2012 Oct 12.
Immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangement (GR) studies have been successfully employed to investigate the clonality and cell lineage of various lymphoid malignancies. Several lymphoma cell lines, such as BJAB, RAJI, DG75 and Jurkat cell lines, were often used as the positive controls in GR detection assays. Of those, the DG75 B-cell lymphoma line was found to exhibit biclonality [two or more homoduplex and heteroduplex bands in a polymerase chain reaction (PCR) product of clonality assay] in the PCR of GR detection assays. To further explore these characteristics of the biclonal phenomenon, the PCR products were purified and cloned into a pEGM-T clone vector. The sequences were analyzed using DNA analysis software. The results demonstrated that the two bands originated from two forms of GR of DG75 cell lines, i.e., DG75 is a biclonal cell line in Ig GRs, which has not been reported before.
免疫球蛋白（Ig）和T细胞受体（TCR）基因重排（GR）研究已成功用于调查各种淋巴恶性肿瘤的克隆性和细胞谱系。几种淋巴瘤细胞系，如BJAB、RAJI、DG75和Jurkat细胞系，经常被用作GR检测试验中的阳性对照。其中，在GR检测试验的PCR中，发现DG75 B细胞淋巴瘤系表现出双克隆性[在克隆性检测试验的聚合酶链反应（PCR）产物中有两条或更多条同源双链和异源双链条带]。为了进一步探索双克隆现象的这些特征，将PCR产物纯化并克隆到pEGM-T克隆载体中。使用DNA分析软件对序列进行分析。结果表明，这两条条带源自DG75细胞系的两种GR形式，即DG75在Ig GR中是双克隆细胞系，这在以前尚未见报道。
