Jiang Qian, Qiu Ya-Ting, Chen Min-Jie, Zhang Zhi-Yuan, Yang Chi
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, P.R. China.
Biomed Rep. 2013 Mar;1(2):218-222. doi: 10.3892/br.2012.41. Epub 2012 Nov 26.
Osteoarthritis (OA) is a slow progressing degenerative disease that affects the joints, including the temporomandibular joint. In the present study, transforming growth factor-β1 (TGF-β1) and matrix metalloproteinase 3 (MMP-3) in synovial fluid (SF) were examined in detecting cartilage synthesis and degradation in progression of temporomandibular joint osteoarthritis (TMJ OA) combined with disc displacement (DD) diseases. SF was obtained from 16 patients with TMJ OA combined with DD and 10 normal volunteers. TGF-β1 and MMP-3 levels were measured by enzyme-linked immunosorbent assay. In addition, TMJ OA combined with DD was classified into three stages based on radiographic signs on the preoperative tomograms and surgical findings at operation, and different treatment options were administered according to the stages. SF from TMJs with TMJ OA combined with DD showed higher levels of TGF-β1 and MMP-3 compared with the asymptomatic control TMJs. With the progression of TMJ OA combined with DD, TGF-β1 levels in SF were lower, while MMP-3 levels in SF were significantly higher. In conclusion, these data suggest that MMP-3 is not only involved in the pathological destruction process of TMJ OA combined with DD initially, but also has a positive correlation with the degree of pathological changes. Furthermore, a significant increase of TGF-β1 levels was found in the SF that were able to counteract the deleterious effects of MMP-3 at the early stage of TMJ OA combined DD, providing the scientific basis on repositioning displaced disc as early as possible for these patients.
骨关节炎（OA）是一种影响关节（包括颞下颌关节）的缓慢进展性退行性疾病。在本研究中，检测了颞下颌关节骨关节炎（TMJ OA）合并盘移位（DD）疾病进展过程中滑液（SF）中的转化生长因子-β1（TGF-β1）和基质金属蛋白酶3（MMP-3），以评估软骨合成和降解情况。从16例TMJ OA合并DD患者和10名正常志愿者中获取滑液。采用酶联免疫吸附测定法测量TGF-β1和MMP-3水平。此外，根据术前断层扫描的影像学征象和手术中的手术发现，将TMJ OA合并DD分为三个阶段，并根据阶段给予不同的治疗方案。与无症状对照颞下颌关节相比，TMJ OA合并DD的颞下颌关节滑液中TGF-β1和MMP-3水平更高。随着TMJ OA合并DD的进展，滑液中TGF-β1水平降低，而MMP-3水平显著升高。总之，这些数据表明，MMP-3不仅最初参与TMJ OA合并DD的病理破坏过程，而且与病理变化程度呈正相关。此外，在TMJ OA合并DD早期的滑液中发现TGF-β1水平显著升高，能够抵消MMP-3的有害作用，为这些患者尽早复位移位盘提供了科学依据。
